ArticleHeliyon2020
Phylogenetic analysis and epidemiological history of Hepatitis E virus 3f and 3c in swine and wild boar, Italy.
Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Genetic Heterogeneity of Hepatitis E Virus (HEV) in Wild Boars in Italy.Animals : an open access journal from MDPI · 2026Article
- Article
- HEV-3 subtypes and strains detected in cases of HEV infection in central Italy from 2015 to 2023.Infection · 2025Article
- Hepatitis E Virus RNA Detection in Liver and Muscle Tissues Sampled from Home Slaughtered Domestic Pigs in Central Italy.Food and environmental virology · 2024Article
- Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy.Viruses · 2023Article
- Hepatitis E Virus in the Wild Boar Population: What Is the Real Zoonotic Risk in Portugal?Tropical medicine and infectious disease · 2023Article
- Temporal changes in the genotypes of Paslahepevirus balayani in southern Spain and their possible link with changes in pig trade imports.One health (Amsterdam, Netherlands) · 2023Article
- The Role of Wildlife and Pests in the Transmission of Pathogenic Agents to Domestic Pigs: A Systematic Review.Animals : an open access journal from MDPI · 2023Review
- Hepatitis E Virus (HEV): Identification of Subtypes 3b and 3m in Wild Boar Population in Liguria Region, Italy.Pathogens (Basel, Switzerland) · 2022Article
- Hepatitis E Virus Detection in Hunted Wild Boar Liver and Muscle Tissues in Central Italy.Microorganisms · 2022Article
- Hepatitis E virus detection in hunted wild boar (Italian journal of food safety · 2022Article
- Molecular Identification and Characterization of a Genotype 3 Hepatitis E Virus (HEV) Strain Detected in a Wolf Faecal Sample, Italy.Animals : an open access journal from MDPI · 2021Article
- Hepatitis E Virus (HEV) Spread and Genetic Diversity in Game Animals in Northern Italy.Food and environmental virology · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHepatitis E virus (HEV) genotype 3 has a worldwide distribution. The food-borne transmission of HEV associated with the consumption of products derived from domestic pig, wild boar has been reported in various countries. In this study the genetic diversity, evolutionary rates of HEV 3f, 3c among swine and wild boar in Italy were estimated.
methodsSampling was performed on a wild boar population living in an area located in Abruzzo region. The HEV RNA amplification was performed by real-time RT-PCR. Nested RT-PCR and sequencing of the ORF2 region were carried out by the Super Script III First-Strand Synthesis System. Sequencing of purified PCR products was carried out by the Genome Lab Dye Terminator Cycle Sequencing (DTCS) Quick Start Kit. The maximum likelihood trees were generated by using Phyml. The mean evolutionary rates and the dated trees were co-estimated by BEAST.
resultsThe phylogenetic analysis showed that the HEV ORF2 isolates from Abruzzo region belonged to 3f subtype. The prevalent subtypes in Italy were those belonging to 3f and 3c. The estimated mean values of the HEV ORF2 capsid gene evolutionary rates were 1.915 × 10
conclusionOur study provide insight into the evolution, circulation of HEV 3f and 3c in Italy.Continued genomic surveillance of HEV in animal reservoir, as well as improving sanitary control measures are required.
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