ArticleScientific reports2020
New 12S metabarcoding primers for enhanced Neotropical freshwater fish biodiversity assessment.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Enhancing the 12S rRNA reference library of South African chondrichthyans to aid in eDNA metabarcoding.BMC zoology · 2026Article
- Refinement and application of 12SCurrent research in food science · 2026Article
- DNA Barcoding of Teleost Fishes from North Luzon, Philippines: A Dataset for Ichthyofaunal Diversity Assessment.Scientific data · 2025Article
- Hydrological Connectivity Enhances Fish Biodiversity in Amazonian Mining Ponds: Insights From eDNA and Traditional Sampling.Molecular ecology · 2025Article
- Environmental DNA illuminates the darkness of mesophotic assemblages of fishes from West Indian Ocean.PloS one · 2025Article
- Checklist of ichthyoplankton of NORI-D polymetallic nodule exploration claim (eastern Clarion-Clipperton Zone) during winter 2021.Biodiversity data journal · 2025Article
- Multi-method survey rediscovers critically endangered species and strengthens Madagascar's freshwater fish conservation.Scientific reports · 2024Article
- Identification of non-model mammal species using the MinION DNA sequencer from Oxford Nanopore.PeerJ · 2024Article
- DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker-dependent.Ecology and evolution · 2023Article
- Sensitive and accurate DNA metabarcoding of parasitic helminth mock communities using the mitochondrial rRNA genes.Scientific reports · 2022Article
- Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary.PloS one · 2022Article
- Life barcoded by DNA barcodes.Conservation genetics resources · 2022Review
- Monitoring fish communities through environmental DNA metabarcoding in the fish pass system of the second largest hydropower plant in the world.Scientific reports · 2021Article
- The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The megadiverse Neotropical fish fauna lacks a comprehensive and reliable DNA reference database, which hampers precise species identification and DNA based biodiversity assessment in the region. Here, we developed a mitochondrial 12S ribosomal DNA reference database for 67 fish species, representing 54 genera, 25 families, and six major Neotropical orders. We aimed to develop mini-barcode markers (i.e. amplicons with less than 200 bp) suitable for DNA metabarcoding by evaluating the taxonomic resolution of full-length and mini-barcodes and to determine a threshold value for fish species delimitation using 12S. Evaluation of the target amplicons demonstrated that both full-length library (565 bp) and mini-barcodes (193 bp) contain enough taxonomic resolution to differentiate all 67 fish species. For species delimitation, interspecific genetic distance threshold values of 0.4% and 0.55% were defined using full-length and mini-barcodes, respectively. A custom reference database and specific mini-barcode markers are important assets for ecoregion scale DNA based biodiversity assessments (such as environmental DNA) that can help with the complex task of conserving the megadiverse Neotropical ichthyofauna.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.