ArticleProceedings of the National Academy of Sciences of the United States of America2020
Mosquito metabolomics reveal that dengue virus replication requires phospholipid reconfiguration via the remodeling cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Multiple orthoflaviviruses secrete sfRNA in mosquito saliva to promote transmission by inhibiting MDA5-mediated early interferon response.Nature communications · 2026Article
- Review
- iPLA2β-dependent phospholipid remodeling drives ZIKV-induced neuroinflammation and neurological injury.Journal of neuroinflammation · 2026Article
- Insect Lipid Metabolism in the Presence of Symbiotic and Pathogenic Viruses and Bacteria.Advances in experimental medicine and biology · 2026Review
- The inhibition mechanism ofFrontiers in microbiology · 2026Review
- Insect Lipidomics: Advances, Applications, and Physiological Insights.Advances in experimental medicine and biology · 2026Review
- Dengue virus and lipid metabolism: unravelling the interplay for future therapeutic approaches.Emerging microbes & infections · 2025Review
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells.Scientific reports · 2025Article
- Reduced microbe abundance in an urban larval development container increases Aedes aegypti susceptibility to Zika virus.PLoS pathogens · 2025Article
- Article
- West Nile virus can be transmitted within mosquito populations through infectious mosquito excreta.iScience · 2024Article
- Glycerophospholipid remodeling is critical for orthoflavivirus infection.Nature communications · 2024Article
- Bloodmeals fuel dengue virus replication in the female mosquitoJournal of virology · 2024Article
- Differential intra-host infection kinetics inmSphere · 2023Article
- The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence.Communications biology · 2023Article
- Profiling lipidomic changes in dengue-resistant and dengue-susceptible strains of Colombian Aedes aegypti after dengue virus challenge.PLoS neglected tropical diseases · 2023Article
- The influence of the larval microbiome on susceptibility to Zika virus is mosquito genotype-dependent.PLoS pathogens · 2023Article
- The buzz in the field: the interaction between viruses, mosquitoes, and metabolism.Frontiers in cellular and infection microbiology · 2023Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue virus (DENV) subdues cell membranes for its cellular cycle by reconfiguring phospholipids in humans and mosquitoes. Here, we determined how and why DENV reconfigures phospholipids in the mosquito vector. By inhibiting and activating the de novo phospholipid biosynthesis, we demonstrated the antiviral impact of de novo-produced phospholipids. In line with the virus hijacking lipids for its benefit, metabolomics analyses indicated that DENV actively inhibited the de novo phospholipid pathway and instead triggered phospholipid remodeling. We demonstrated the early induction of remodeling during infection by using isotope tracing in mosquito cells. We then confirmed in mosquitoes the antiviral impact of de novo phospholipids by supplementing infectious blood meals with a de novo phospholipid precursor. Eventually, we determined that phospholipid reconfiguration was required for viral genome replication but not for the other steps of the virus cellular cycle. Overall, we now propose that DENV reconfigures phospholipids through the remodeling cycle to modify the endomembrane and facilitate formation of the replication complex. Furthermore, our study identified de novo phospholipid precursor as a blood determinant of DENV human-to-mosquito transmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.