Observational studyBlood transfusion = Trasfusione del sangue2021
Immediate effects of whole blood donation on the endothelial surface layer and glycocalyx shedding.
Observational study in Blood transfusion = Trasfusione del sangue, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
8 citing papers in PubMed.
- Endothelial glycocalyx during early reperfusion in patients undergoing cardiac surgery.PloS one · 2021Trial
- Sublingual Sidestream Dark Field (SDF) Microscopy With GlycoCheck Analysis in Individuals With and Without Cardiovascular Risk Factors and Disease.Journal of the American Heart Association · 2026Article
- Effect on Syndecan-1 and Hyaluronan Levels Depending on Multiple Organ Failure, Coagulopathy and Survival: An Observational Study in Major Trauma Patients.Journal of clinical medicine · 2024Article
- The Endothelial Glycocalyx in Pig-to-Baboon Cardiac Xenotransplantation-First Insights.Biomedicines · 2024Article
- Insights into the Molecular Mechanism of Endothelial Glycocalyx Dysfunction during Heart Surgery.Current issues in molecular biology · 2024Review
- Endothelial Dysfunction in Patients Undergoing Cardiac Surgery: A Narrative Review and Clinical Implications.Journal of cardiovascular development and disease · 2023Review
- Microvascular perfusion, perfused boundary region and glycocalyx shedding in patients with autosomal dominant polycystic kidney disease: results from the GlycoScore III study.Clinical kidney journal · 2023Article
- Effects of a single aerobic exercise on perfused boundary region and microvascular perfusion: a field study.Journal of clinical monitoring and computing · 2022Observational
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe endothelium plays a pivotal role in regulating microvascular function, especially in situations associated with acute blood loss. Whether blood donation and the associated volume loss affects the dimensions of the endothelial surface layer (ESL) and glycocalyx integrity remains unknown. MATERIALS AND
methodsThis study was designed to determine real-time ESL and perfusion measurements of the sublingual microcirculation using sidestream dark field imaging performed in healthy subjects shortly before and after a donation of 500 mL whole blood. A novel image acquisition and analysis software (GlycoCheck™) automatically calculated the perfused boundary region (PBR), an inverse parameter for red blood cell (RBC) penetration into the ESL, in vessels between 5 and 25 μm in diameter. Microvascular perfusion was measured by RBC filling percentage. Soluble glycocalyx components were determined in the peripheral circulation.
resultsThere was no significant immediate effect of whole blood donation on PBR and RBC filling percentage. Linear regression analysis revealed a distinct association between change in PBR and change in RBC filling percentage (regression coefficient β: -0.040; 95% confidence interval: -0.049 to -0.030; p<0.001). A significant reduction in plasma heparan sulphate (1,329±316 vs 1,237±275 ng/mL, p=0.005) and hyaluronan (94±18 vs 90±16 ng/mL, p=0.002) was noted, while syndecan-1 levels (30 [23-50] vs 29 [24-46] ng/mL, p=0.282) remained unchanged. DISCUSSION: Dimensions and integrity of the ESL appear to remain stable following a 500 mL whole blood donation and reflect its ability to ensure microvascular function and perfusion.
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