ArticlePlant cell reports2021
IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.).
Article in Plant cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- First systematic review of the last 30 years of research on sweetpotato: elucidating the frontiers and hotspots.Frontiers in plant science · 2024Pooled it
- Elucidating Scent and Color Variation in White and Pink-FloweredPlants (Basel, Switzerland) · 2026Article
- Molecular mechanism of ethylene signaling response gene VcERF061 to promote anthocyanin synthesis in blueberry (Vaccinium corymbosum).BMC plant biology · 2025Article
- Integrative analysis of the transcriptome and metabolome reveals the peel coloration mechanism of Zanthoxylum armatum.BMC plant biology · 2025Article
- Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato.BMC plant biology · 2024Article
- Article
- Effect of Growth Stages on Anthocyanins and Polyphenols in the Root System of Sweet Potato.Plants (Basel, Switzerland) · 2023Article
- Anthocyanins in Plant Food: Current Status, Genetic Modification, and Future Perspectives.Molecules (Basel, Switzerland) · 2023Review
- Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes.BMC plant biology · 2022Article
- Genome-wide identification and expression analysis of the bHLH gene family in cauliflower (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in 'Kuerle Xiangli' (Genes · 2022Article
- Anthocyanin-Rich Vegetables for Human Consumption-Focus on Potato, Sweetpotato and Tomato.International journal of molecular sciences · 2022Review
- Integrated metabolomic and transcriptomic analyses reveal molecular response of anthocyanins biosynthesis in perilla to light intensity.Frontiers in plant science · 2022Article
- The Transcription Factor MdERF78 Is Involved in ALA-Induced Anthocyanin Accumulation in Apples.Frontiers in plant science · 2022Article
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Abstract
key messageThe transcription factor (TF) IbERF71 forms a novel complex, IbERF71-IbMYB340-IbbHLH2, to coregulate anthocyanin biosynthesis by binding to the IbANS1 promoter in purple-fleshed sweet potatoes. Purple-fleshed sweet potato (Ipomoea batatas L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TF IbERF71 and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels of IbERF71 and partners IbMYB340 and IbbHLH2 were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation of IbMYB340+IbbHLH2 resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additional IbERF71 significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels of FvANS and FvGST, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity of IbANS1. In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes.
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