ArticleiScience2020
Trans-omic Analysis Reveals ROS-Dependent Pentose Phosphate Pathway Activation after High-Frequency Electrical Stimulation in C2C12 Myotubes.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Skeletal muscle hypertrophy rewires glucose metabolism: An experimental investigation and systematic review.Journal of cachexia, sarcopenia and muscle · 2024Pooled it
- Investigating Coenzyme Function of Thiamine Triphosphate Using Its Novel Hydrolysis-Resistant Analog and Transketolase.Biomolecules · 2026Article
- In vitro muscle contraction: A technical review on electrical pulse stimulation in C2C12 cells.Experimental physiology · 2025Article
- Metabolic dynamics of human external urethral sphincter myoblast differentiation and the effects of tricarboxylic acid cycle inhibition.Scientific reports · 2025Article
- Constitutively active mTORC1 signaling modifies the skeletal muscle metabolome and lipidome response to exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2025Article
- Interaction of the C2C12 myotube contractions and glucose availability on transcriptome and extracellular vesicle microRNAs.American journal of physiology. Cell physiology · 2024Article
- Periodontal treatment and microbiome-targeted therapy in management of periodontitis-related nonalcoholic fatty liver disease with oral and gut dysbiosis.World journal of gastroenterology · 2023Review
- Article
- Does a Hypertrophying Muscle Fibre Reprogramme its Metabolism Similar to a Cancer Cell?Sports medicine (Auckland, N.Z.) · 2022Review
- Review
- Article
- Evidence of myomiR regulation of the pentose phosphate pathway during mechanical load-induced hypertrophy.Physiological reports · 2021Article
- Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation.American journal of physiology. Endocrinology and metabolism · 2021Article
- Multiblock metabolomics: An approach to elucidate whole-body metabolism with multiblock principal component analysis.Computational and structural biotechnology journal · 2021Article
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skeletal muscle adaptation is mediated by cooperative regulation of metabolism, signal transduction, and gene expression. However, the global regulatory mechanism remains unclear. To address this issue, we performed electrical pulse stimulation (EPS) in differentiated C2C12 myotubes at low and high frequency, carried out metabolome and transcriptome analyses, and investigated phosphorylation status of signaling molecules. EPS triggered extensive and specific changes in metabolites, signaling phosphorylation, and gene expression during and after EPS in a frequency-dependent manner. We constructed trans-omic network by integrating these data and found selective activation of the pentose phosphate pathway including metabolites, upstream signaling molecules, and gene expression of metabolic enzymes after high-frequency EPS. We experimentally validated that activation of these molecules after high-frequency EPS was dependent on reactive oxygen species (ROS). Thus, the trans-omic analysis revealed ROS-dependent activation in signal transduction, metabolome, and transcriptome after high-frequency EPS in C2C12 myotubes, shedding light on possible mechanisms of muscle adaptation.
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