ArticlePeerJ2020
An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9.
Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- Toward Breeding by Gene Design: Constructing the Ideotype ofPlants (Basel, Switzerland) · 2026Review
- Multiplex CRISPR-Cas9 editing of chlorophyll biosynthesis genes in chickpea via protoplast and Agrobacterium-mediated transformation.Functional & integrative genomics · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Salt stress-induced remodeling of sugar transport: a role for promoter alleles of SWEET13.Scientific reports · 2025Article
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- Uncovering the key lncRNAs in regulating cadmium accumulation and translocation in sweet sorghum.Planta · 2024Article
- A streamlined guide RNA screening system for genome editing in Sorghum bicolor.Plant methods · 2023Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation.Nature communications · 2023Article
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.Plant methods · 2022Article
- Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review.Plants (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protoplasts are commonly used in genetic and breeding research. In this study, the isolation of sorghum protoplasts was optimized and applied to transient gene expression and editing by CRISPR/Cas9. The protoplast was most viable in 0.5 M mannitol, which was the highest of three concentrations after 48- and 72-hours treatments. Using this method we can derive an average of 1.6×10
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Registered trials
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