ArticlemSystems2020
A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in Mycobacterium abscessus.
Article in mSystems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Transposon-based genome editing of industrial microorganisms: advances, challenges, and prospects.Synthetic and systems biotechnology · 2026Review
- GplR1, an unusual TetR-like transcription factor inmSystems · 2025Article
- Transposon-directed insertion-site sequencing (TraDIS) analysis ofMicrobiology spectrum · 2025Article
- Transposon-sequencing across multiplemBio · 2025Article
- Investigating the role ofMicrobiology spectrum · 2024Article
- Loss of LpqM proteins inMicrobiology spectrum · 2024Article
- Identification of genes associated with persistence inFrontiers in microbiology · 2024Article
- Chemical genetic approaches for the discovery of bacterial cell wall inhibitors.RSC medicinal chemistry · 2023Review
- The small non-coding RNA B11 regulates multiple facets of Mycobacterium abscessus virulence.PLoS pathogens · 2023Article
- pBLAM1-x: standardized transposon tools for high-throughput screening.Synthetic biology (Oxford, England) · 2023Article
- Transposon mutagenesis ineLife · 2022Article
- Comparison of Isogenic Strains Shows No Evidence of Altered Nosocomial Transmission-Competency of Rough, GPL-Negative Mycobacterium abscessus Strains.Microbiology spectrum · 2022Article
- Genome-Wide Essentiality Analysis ofmBio · 2021Article
- Rifabutin Is Inactivated by Mycobacterium abscessus Arr.Antimicrobial agents and chemotherapy · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and straightforward identification of the insertion site, by generating a longer sequence surrounding the insertion TA in the genome. The method makes Tn-seq more user-friendly and accessible to laboratories with more-limited bioinformatic resources. Finally, we created a saturated transposon-mutant library in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.