ArticlemBio2020
Environmental and Physiological Factors Affecting High-Throughput Measurements of Bacterial Growth.
Article in mBio, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
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- Environmental cues shape extracellular vesicles biogenesis and function in Streptococcus pneumoniae.BMC microbiology · 2026Article
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- Time Series Data Mining for Linking the Shape of Bacterial Growth Curves to Biological Functions.Computational and structural biotechnology journal · 2026Article
- Maltose aids in the growth ofFEMS microbes · 2026Article
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- Experimental Challenge of Chinook Salmon (Oncorhynchus tshawytscha) With Tenacibaculum maritimum and Tenacibaculum dicentrarchi Fulfils Koch's Postulates.Journal of fish diseases · 2025Article
- Short- and Long-Term Evolution of Microbial Cultures: A Thermodynamic Perspective.International journal of molecular sciences · 2025Article
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- Metabolic rearrangement enables adaptation of microbial growth rate to temperature shifts.Nature microbiology · 2025Article
- Trace elements increase replicability of microbial growth.Open biology · 2025Article
- Flagellar point mutation causes social aggregation in laboratory-adaptedJournal of bacteriology · 2024Article
- Cultivating efficiency: high-throughput growth analysis of anaerobic bacteria in compact microplate readers.Microbiology spectrum · 2024Article
- Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries.Microbial biotechnology · 2024Article
- Resource competition predicts assembly of gut bacterial communities in vitro.Nature microbiology · 2024Article
- Microbial Primer: Bacterial growth kinetics.Microbiology (Reading, England) · 2024Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Bacterial growth under nutrient-rich and starvation conditions is intrinsically tied to the environmental history and physiological state of the population. While high-throughput technologies have enabled rapid analyses of mutant libraries, technical and biological challenges complicate data collection and interpretation. Here, we present a framework for the execution and analysis of growth measurements with improved accuracy over that of standard approaches. Using this framework, we demonstrate key biological insights that emerge from consideration of culturing conditions and history. We determined that quantification of the background absorbance in each well of a multiwell plate is critical for accurate measurements of maximal growth rate. Using mathematical modeling, we demonstrated that maximal growth rate is dependent on initial cell density, which distorts comparisons across strains with variable lag properties. We established a multiple-passage protocol that alleviates the substantial effects of glycerol on growth in carbon-poor media, and we tracked growth rate-mediated fitness increases observed during a long-term evolution of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.