Evidence map›Paper›PMID 33081847›Full record

ArticleActa neuropathologica communications2020

Identification of a dysfunctional microglial population in human Alzheimer's disease cortex using novel single-cell histology image analysis.

Molly E V Swanson, Emma L Scotter, Leon C D Smyth, Helen C Murray, Brigid Ryan, Clinton Turner, Richard L M Faull, Mike Dragunow, Maurice A Curtis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta neuropathologica communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed, 1 pooled it
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 1 synthesis or guideline pooled it, 95 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Increased circulating TREM2Brain, behavior, & immunity - health · 2026
    Article
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  7. Review
  8. Review
  9. Article
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  14. Article
  15. Article
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  17. Early and progressive retinal microglial changes in APPFrontiers in aging neuroscience · 2025
    Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Molly E V SwansonDepartment of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.ORCID 0000-0002-4381-5520
Emma L ScotterCentre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Leon C D SmythCentre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Helen C MurrayDepartment of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Brigid RyanDepartment of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Clinton TurnerDepartment of Anatomical Pathology, LabPlus, Auckland City Hospital, Auckland, New Zealand.
Richard L M FaullDepartment of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Mike DragunowCentre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Maurice A CurtisDepartment of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand. m.curtis@auckland.ac.nz.
University of Auckland · NZAuckland City Hospital · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In Alzheimer's disease (AD), microglia are affected by disease processes, but may also drive pathogenesis. AD pathology-associated microglial populations have been identified with single-cell RNA-Seq, but have not been validated in human brain tissue with anatomical context. Here, we quantified myeloid cell markers to identify changes in AD pathology-associated microglial populations. We performed fluorescent immunohistochemistry on normal (n = 8) and AD (n = 8) middle temporal gyri, co-labelling the pan-myeloid cell marker, Iba1, with one of 11 markers of interest (MOIs): CD45, HLA-DR, CD14, CD74, CD33, CD206, CD32, CD163, P2RY12, TMEM119, L-Ferritin. Novel image analyses quantified the single-cell abundance of Iba1 and each MOI. Each cell was gated into one Iba1-MOI population (Iba1

Indexed as

AgedAged, 80 and overAlzheimer DiseaseAntigens, CDAntigens, Differentiation, B-LymphocyteAntigens, Differentiation, MyelomonocyticCD163 AntigenCerebral CortexFemaleFerritinsHistocompatibility Antigens Class IIHLA-DR AntigensHumansImmunohistochemistryLectins, C-TypeLeukocyte Common AntigensAntigens, CDAntigens, Differentiation, B-LymphocyteAntigens, Differentiation, MyelomonocyticCD163 AntigenCD320 protein, humanFerritinsHistocompatibility Antigens Class IIHLA-DR Antigensinvariant chainLectins, C-TypeLeukocyte Common AntigensLipopolysaccharide ReceptorsMannose-Binding LectinsMannose ReceptorMembrane ProteinsP2RY12 protein, humanPTPRC protein, humanReceptors, Cell SurfaceReceptors, Purinergic P2Y12Sialic Acid Binding Ig-like Lectin 3Tmem119 protein, humanAlzheimer’s diseaseAmyloid betaDysfunctionImmunohistochemistryMicrogliaSingle-cell analysisTau

Identifiers

PMID33081847
PMCPMC7576851
OpenAlexW3093508648

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.