ArticleBMC biology2020
Bioengineered human skeletal muscle capable of functional regeneration.
Article in BMC biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- A Multi-Institution Biobanking Pipeline for Primary Human Satellite Cells and Fibro-Adipogenic Progenitors.bioRxiv : the preprint server for biology · 2026Article
- Three-dimensional models of skeletal muscle under tension: a systematic review and core outcome set.NPJ Regenerative medicine · 2026Article
- Chestnut (Nutrients · 2025Article
- Engineered myovascular tissues for studies of endothelial/satellite cell interactions.Acta biomaterialia · 2024Article
- Inhibitor of FTO, Rhein, Restrains the Differentiation of Myoblasts and Delays Skeletal Muscle Regeneration.Animals : an open access journal from MDPI · 2024Article
- Extracellular vesicles may provide an alternative detoxification pathway during skeletal muscle myoblast ageing.Journal of extracellular biology · 2024Article
- Article
- Mini-MEndR: a miniaturized 96-well predictive assay to evaluate muscle stem cell-mediated repair.BMC methods · 2024Article
- Advancements in 2D and 3D In Vitro Models for Studying Neuromuscular Diseases.International journal of molecular sciences · 2023Review
- Effect of GelMA Hydrogel Properties on Long-Term Encapsulation and Myogenic Differentiation of CGels (Basel, Switzerland) · 2023Article
- Arsenic disrupts extracellular vesicle-mediated signaling in regenerating myofibers.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Bioprocessing Considerations towards the Manufacturing of Therapeutic Skeletal and Smooth Muscle Cells.Bioengineering (Basel, Switzerland) · 2023Review
- Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Modelling Skeletal Muscle Ageing and RepairJournal of tissue engineering and regenerative medicine · 2023Article
- A conciseFrontiers in cell and developmental biology · 2023Article
- Article
- Engineered skeletal muscle recapitulates human muscle development, regeneration and dystrophy.Journal of cachexia, sarcopenia and muscle · 2022Article
- Myoblast deactivation within engineered human skeletal muscle creates a transcriptionally heterogeneous population of quiescent satellite-like cells.Biomaterials · 2022Article
- Review
- Primary skeletal muscle cells from chronic kidney disease patients retain hallmarks of cachexia in vitro.Journal of cachexia, sarcopenia and muscle · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSkeletal muscle (SkM) regenerates following injury, replacing damaged tissue with high fidelity. However, in serious injuries, non-regenerative defects leave patients with loss of function, increased re-injury risk and often chronic pain. Progress in treating these non-regenerative defects has been slow, with advances only occurring where a comprehensive understanding of regeneration has been gained. Tissue engineering has allowed the development of bioengineered models of SkM which regenerate following injury to support research in regenerative physiology. To date, however, no studies have utilised human myogenic precursor cells (hMPCs) to closely mimic functional human regenerative physiology.
resultsHere we address some of the difficulties associated with cell number and hMPC mitogenicity using magnetic association cell sorting (MACS), for the marker CD56, and media supplementation with fibroblast growth factor 2 (FGF-2) and B-27 supplement. Cell sorting allowed extended expansion of myogenic cells and supplementation was shown to improve myogenesis within engineered tissues and force generation at maturity. In addition, these engineered human SkM regenerated following barium chloride (BaCl
conclusionsThis work outlines an engineered human SkM capable of functional regeneration following injury, built upon an open source system adding to the pre-clinical testing toolbox to improve the understanding of basic regenerative physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.