Evidence map›Paper›PMID 33080923›Full record

ArticleMolecules (Basel, Switzerland)2020

Mass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization.

Bright D Danquah, Kwabena F M Opuni, Claudia Roewer, Cornelia Koy, Michael O Glocker

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Bright D DanquahProteome Center Rostock, University Medicine Rostock, 18059 Rostock, Germany.ORCID 0000-0002-6990-8434
Kwabena F M OpuniSchool of Pharmacy, University of Ghana, P. O. Box LG53 Legon, Ghana.ORCID 0000-0003-1653-1458
Claudia RoewerProteome Center Rostock, University Medicine Rostock, 18059 Rostock, Germany.
Cornelia KoyProteome Center Rostock, University Medicine Rostock, 18059 Rostock, Germany.
Michael O GlockerProteome Center Rostock, University Medicine Rostock, 18059 Rostock, Germany.
University of Rostock · DEUniversity of Ghana · GH

Funding

European Union (EU) EFRE-UHROM 9German Academic Exchange Service (DAAD) 91548123German Academic Exchange Service (DAAD) 91566064
6 · The paper itself

Abstract

Electrospray mass spectrometry is applied to determine apparent binding energies and quasi equilibrium dissociation constants of immune complex dissociation reactions in the gas phase. Myoglobin, a natural protein-ligand complex, has been used to develop the procedure which starts from determining mean charge states and normalized and averaged ion intensities. The apparent dissociation constant KD m0g#= 3.60 × 10

Indexed as

AntibodiesAntigen-Antibody ComplexEpitopesHemeHumansImmunoconjugatesLigandsMultiprotein ComplexesMyoglobinOligopeptidesPeptidesRibonucleasesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAntibodiesAntigen-Antibody ComplexEpitopesFLAG peptideHemeImmunoconjugatesLigandsMultiprotein ComplexesMyoglobinOligopeptidesPeptidesribonuclease SRibonucleasesapparent gas phase activation energiesapparent gas phase dissociation constantsgas phase immune complex dissociationITEM-TWOmass spectrometric epitope mappingnative mass spectrometry

Identifiers

PMID33080923
PMCPMC7587528
OpenAlexW3093192010

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.