Evidence map›Paper›PMID 33079215›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2021

A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea.

Rachit K Saxena, Anil Hake, Abhishek Bohra, Aamir W Khan, Anupama Hingane, Rafat Sultana, Indra Prakash Singh, S J Satheesh Naik, Rajeev K Varshney

Open access · hybridAbstract read
In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Sterility Mosaic Disease of Pigeonpea (Plants (Basel, Switzerland) · 2024
    Review
  4. Article
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Rachit K SaxenaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India. r.saxena@cgiar.org.ORCID http://orcid.org/0000-0002-9405-3570
Anil HakeInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India.
Abhishek BohraICAR- Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India.
Aamir W KhanICAR- Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India.
Anupama HinganeInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India.
Rafat SultanaBihar Agricultural University, Sabour, Bhagalpur, Bihar, India.
Indra Prakash SinghICAR- Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India.
S J Satheesh NaikICAR- Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India.
Rajeev K VarshneyInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India. r.k.varshney@cgiar.org.ORCID http://orcid.org/0000-0002-4562-9131
Indian Institute of Pulses Research · INInternational Crops Research Institute for the Semi-Arid Tropics · INBihar Agricultural University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fusarium wilt (FW) and sterility mosaic diseases (SMD) are key biotic constraints to pigeonpea production. Occurrence of these two diseases in congenial conditions is reported to cause complete yield loss in susceptible pigeonpea cultivars. Various studies to elucidate genomic architecture of the two traits have revealed significant marker-trait associations for use in breeding programs. However, these DNA markers could not be used effectively in genomics-assisted breeding for developing FW and SMD resistant varieties primarily due to pathogen variability, location or background specificity, lesser phenotypic variance explained by the reported QTL and cost-inefficiency of the genotyping assays. Therefore, in the present study, a novel approach has been used to develop a diagnostic kit for identification of suitable FW and SMD resistant lines. This kit was developed with 10 markers each for FW and SMD resistance. Investigation of the diversity of these loci has shown the role of different alleles in different resistant genotypes. Two genes (C.cajan_03691 and C.cajan_18888) for FW resistance and four genes (C.cajan_07858, C.cajan_20995, C.cajan_21801 and C.cajan_17341) for SMD resistance have been identified. More importantly, we developed a customized and cost-effective Kompetitive allele-specific PCR genotyping assay for the identified genes in order to encourage their downstream applications in pigeonpea breeding programs. The diagnostic marker kit developed here will offer great strength to pigeonpea varietal development program, since the resistance against these two diseases is essentially required for nominating an improved line in varietal release pipeline.

Indexed as

AllelesCajanusDisease ResistanceFusariumGenes, PlantGenetic MarkersGenotypeGenotyping TechniquesINDEL MutationPhenotypePlant DiseasesPlant InfertilityPolymorphism, Single NucleotideSelection, GeneticGenetic Markers

Identifiers

PMID33079215
PMCPMC7813729
OpenAlexW3092679407

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.