Evidence map›Paper›PMID 33079213›Full record

ArticleAnalytical and bioanalytical chemistry2021

Integration of magnetic separation and real-time ligation chain reaction for detection of uracil-DNA glycosylase.

Jinying Liu, Jiangyan Zhang, Meiqi Chen, Dehui Qiu, Xuechong Lv, Qi Jiang, Yongqiang Cheng

Abstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 7 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jinying LiuKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Jiangyan ZhangKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. hdzjy2005@163.com.ORCID http://orcid.org/0000-0001-9559-6713
Meiqi ChenKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Dehui QiuKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Xuechong LvKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Qi JiangKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Yongqiang ChengKey Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. yqcheng@hbu.edu.cn.
Hebei University · CN

Funding

the National Natural Science Foundation of China 21605034the Natural Science Foundation of Hebei Province B2018201049
6 · The paper itself

Abstract

Uracil-DNA glycosylase (UDG) is a protein enzyme that initiates the base excision repair pathway for maintaining genome stability. Sensitive detection of UDG activity is important in the study of many biochemical processes and clinical applications. Here, a method for detecting UDG is proposed by integrating magnetic separation and real-time ligation chain reaction (LCR). First, a DNA substrate containing uracil base is designed to be conjugated to the magnetic beads. By introducing a DNA complementary to the DNA substrate, the uracil base is recognized and removed by UDG to form an apurinic/apyrimidinic (AP) site. The DNA substrate is then cut off from the AP site by endonuclease IV, releasing a single-strand DNA (ssDNA). After magnetic separation, the ssDNA is retained in the supernatant and then detected by real-time LCR. The linear range of the method is 5 × 10

Indexed as

HeLa CellsHot TemperatureHumansLigase Chain ReactionLimit of DetectionReproducibility of ResultsUracil-DNA GlycosidaseUracil-DNA GlycosidaseFluorescence detectionLigation chain reactionMagnetic separationReal-time detectionUracil-DNA glycosylase

Identifiers

PMID33079213
OpenAlexW3092857061

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.