Evidence map›Paper›PMID 33078324›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Isolation, characterization, and ecotoxicological application of marine mammal skin fibroblast cultures.

Andrey V Boroda, Yulia O Kipryushina, Raisa V Golochvastova, Olga G Shevchenko, Mariia A Shulgina, Kseniya V Efimova, Igor O Katin, Mariia A Maiorova

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Bridging methodological gaps in avian cytogenetics: comprehensive and optimized protocols for chromosomal preparation in birds.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
    Article
  2. Article
  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Andrey V BorodaNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia. avboroda@imb.dvo.ru.ORCID http://orcid.org/0000-0003-0257-748X
Yulia O KipryushinaNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.
Raisa V GolochvastovaNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.
Olga G ShevchenkoNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.
Mariia A ShulginaNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.
Kseniya V EfimovaNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.
Igor O KatinNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.
Mariia A MaiorovaNational Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevsky St, 690041, Vladivostok, Russia.

Funding

The grant of the President of Russian Federation for young scientists МК-264.2017.4Российский Фонд Фундаментальных Исследований (РФФИ) 19-04-00752
6 · The paper itself

Abstract

Marine mammal cell cultures are a multifunctional instrument for acquiring knowledge about life in the world's oceans in physiological, biochemical, genetic, and ecotoxicological aspects. We succeeded in isolation, cultivation, and characterization of skin fibroblast cultures from five marine mammal species. The cells of the spotted seal (Phoca largha), the sea lion (Eumetopias jubatus), and the walrus (Odobenus rosmarus) are unpretentious to the isolation procedure. The sea otter (Enhydra lutris) fibroblasts should be isolated by trypsin disaggregation, while only mechanical disaggregation was suitable for the beluga whale (Delphinapterus leucas) cells. The cell growth parameters have been determined allowing us to find the optimal seeding density for continuous and effective cultivation. The effects of nonpathogenic algal extracts on proliferation, viability, and functional activity of marine mammal cells in vitro have been presented and discussed for the first time.

Indexed as

AnimalsAquatic OrganismsCell ProliferationCells, CulturedCell SeparationDNA, MitochondrialEcotoxicologyFemaleFibroblastsMaleMammalsDNA, MitochondrialAlgal cytotoxicityCell culture parametersFlow cytometryMarine mammalsSkin fibroblast culture

Identifiers

PMID33078324

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.