ArticleAnalytical and bioanalytical chemistry2021
CpG preconditioning reduces accumulation of lysophosphatidylcholine in ischemic brain tissue after middle cerebral artery occlusion.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 27 citations in OpenAlex.
- Spatial regulation effects of Taohong Siwu decoction on disordered lipids in a rat model of ischemic stroke assessed using mass spectrometry imaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Mass spectrometry-based spatiotemporal characterization of lipids in early focal cerebral ischemia.Analytical and bioanalytical chemistry · 2025Article
- Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging (nano-DESI MSI): A Tutorial Review.ACS measurement science au · 2024Review
- Exploring Lysophosphatidylcholine as a Biomarker in Ischemic Stroke: The Plasma-Brain Disjunction.International journal of molecular sciences · 2024Article
- Spatial lipidomics maps brain alterations associated with mild traumatic brain injury.Frontiers in chemistry · 2024Article
- High-resolution integrated microfluidic probe for mass spectrometry imaging of biological tissues.Analytica chimica acta · 2023Article
- Ischemic Stroke Causes Disruptions in the Carnitine Shuttle System.Metabolites · 2023Article
- Host-Guest Chemistry for Simultaneous Imaging of Endogenous Alkali Metals and Metabolites with Mass Spectrometry.Analytical chemistry · 2022Article
- Silver-Doped Nano-DESI MSI for Increased Specificity and Sensitivity of Alkenes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Guhong Injection Alleviates Cerebral Ischemia-Reperfusion Injury via the PKC/HIF-1α Pathway in Rats.Frontiers in pharmacology · 2021Article
- Eriodictyol Attenuates MCAO-Induced Brain Injury and Neurological DeficitsFrontiers in systems neuroscience · 2021Article
- An imbalanced ratio between PC(16:0/16:0) and LPC(16:0) revealed by lipidomics supports the role of the Lands cycle in ischemic brain injury.The Journal of biological chemistryArticle
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Authors and funding
6 authors at 3 institutions in 2 countries.
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Abstract
Ischemic stroke is one of the major causes of death and permanent disability in the world. However, the molecular mechanisms surrounding tissue damage are complex and further studies are needed to gain insights necessary for development of treatment. Prophylactic treatment by administration of cytosine-guanine (CpG) oligodeoxynucleotides has been shown to provide neuroprotection against anticipated ischemic injury. CpG binds to Toll-like receptor 9 (TLR9) causing initialization of an inflammatory response that limits visible ischemic damages upon subsequent stroke. Here, we use nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging (MSI) to characterize molecular effects of CpG preconditioning prior to middle cerebral artery occlusion (MCAO) and reperfusion. By doping the nano-DESI solvent with appropriate internal standards, we can study and compare distributions of phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) in the ischemic hemisphere of the brain despite the large changes in alkali metal abundances. Our results show that CpG preconditioning not only reduces the infarct size but it also decreases the degradation of PC and accumulation of LPC species, which indicates reduced cell membrane breakdown and overall ischemic damage. Our findings show that molecular mechanisms of PC degradation are intact despite CpG preconditioning but that these are limited due to the initialized inflammatory response.
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