Evidence map›Paper›PMID 33078202›Full record

ArticleInternational journal of colorectal disease2021

CIP2A regulates MYC translation (via its 5'UTR) in colorectal cancer.

S Denk, S Schmidt, Y Schurr, G Schwarz, F Schote, M Diefenbacher, C Armendariz, F Dejure, M Eilers, Armin Wiegering

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of colorectal disease, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Genome-wide transcriptome analysis ofFrontiers in cellular and infection microbiology · 2024
    Article
  4. Article
  5. The Functional Meaning of 5'UTR in Protein-Coding Genes.International journal of molecular sciences · 2023
    Review
  6. Review
  7. Significant position of C-myc in colorectal cancer: a promising therapeutic target.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2022
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

S DenkDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
S SchmidtDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
Y SchurrDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
G SchwarzDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
F SchoteDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
M DiefenbacherDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
C ArmendarizDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
F DejureDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
M EilersDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany.
Armin WiegeringDepartment of Biochemistry and Molecular Biology, Biocenter, University of Würzburg, Würzburg, Germany. wiegering_a@ukw.de.ORCID http://orcid.org/0000-0001-7777-0909
University of Würzburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDeregulated expression of MYC is a driver of colorectal carcinogenesis, suggesting that decreasing MYC expression may have significant therapeutic value. CIP2A is an oncogenic factor that regulates MYC expression. CIP2A is overexpressed in colorectal cancer (CRC), and its expression levels are an independent marker for long-term outcome of CRC. Previous studies suggested that CIP2A controls MYC protein expression on a post-transcriptional level.

methodsTo determine the mechanism by which CIP2A regulates MYC in CRC, we dissected MYC translation and stability dependent on CIP2A in CRC cell lines.

resultsKnockdown of CIP2A reduced MYC protein levels without influencing MYC stability in CRC cell lines. Interfering with proteasomal degradation of MYC by usage of FBXW7-deficient cells or treatment with the proteasome inhibitor MG132 did not rescue the effect of CIP2A depletion on MYC protein levels. Whereas CIP2A knockdown had marginal influence on global protein synthesis, we could demonstrate that, by using different reporter constructs and cells expressing MYC mRNA with or without flanking UTR, CIP2A regulates MYC translation. This interaction is mainly conducted by the MYC 5'UTR.

conclusionsThus, instead of targeting MYC protein stability as reported for other tissue types before, CIP2A specifically regulates MYC mRNA translation in CRC but has only slight effects on global mRNA translation. In conclusion, we propose as novel mechanism that CIP2A regulates MYC on a translational level rather than affecting MYC protein stability in CRC.

Indexed as

Colorectal Neoplasms5' Untranslated RegionsAutoantigensCell Line, TumorCell ProliferationGene Expression Regulation, NeoplasticHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsProto-Oncogene Proteins c-myc5' Untranslated RegionsAutoantigensCIP2A protein, humanIntracellular Signaling Peptides and ProteinsMembrane ProteinsMYC protein, humanProto-Oncogene Proteins c-mycCIP2AColon cancerMYCTranslation

Identifiers

PMID33078202
PMCPMC8178152
OpenAlexW3093325775

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.