ArticleScientific reports2020
A pioneer calf foetus microbiome.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Modulation of Early-Life Calf Gut Microbiota by Bacteriocin-Producing Lactiplantibacillus pentosus AA86 and Pediococcus pentosaceus AA94.MicrobiologyOpen · 2026Article
- Maternal Microbial Reservoirs Are Associated with Early Bacterial and Archaeal Community Assembly in Neonatal Hu Lambs.Animals : an open access journal from MDPI · 2026Article
- Unraveling the dynamic changes in the intestinal microbiome: impacts on pre-weaning calf health and productivity.Journal of animal science and biotechnology · 2026Review
- Article
- Microbial colonisation of the neonatal beef calf gastrointestinal tract: limited influence of birth delivery method across five anatomical regions.Frontiers in microbiology · 2026Article
- Effects ofAnimals : an open access journal from MDPI · 2025Article
- Optimizing bacteriophage screening and isolation methods for microbial samples derived from different body sites of cattle.bioRxiv : the preprint server for biology · 2025Article
- The influence of prenatal nutrition on ruminal microbiome of beef cattle.Frontiers in genetics · 2025Article
- Effects of dietary restriction and one-carbon metabolite supplementation during the first 63 days of gestation on the maternal gut, vaginal, and blood microbiota in cattle.Animal microbiome · 2024Article
- Meconium microbiota in naturally delivered canine puppies.BMC veterinary research · 2024Article
- Article
- Dynamic Analysis of Stool Microbiota of Simmental Calves and Effects of Diarrhea on Their Gut Microbiota.Biology · 2024Article
- Bacteria colonization and gene expression related to immune function in colon mucosa is associated with growth in neonatal calves regardless of live yeast supplementation.Journal of animal science and biotechnology · 2024Article
- Life at the borderlands: microbiomes of interfaces critical to One Health.FEMS microbiology reviews · 2024Review
- Bovine reproductive tract and microbiome dynamics: current knowledge, challenges, and its potential to enhance fertility in dairy cows.Frontiers in microbiomes · 2024Review
- The dogma of the sterile uterus revisited: does microbial seeding occur during fetal life in humans and animals?Reproduction (Cambridge, England) · 2024Review
- Health-associated changes of the fecal microbiota in dairy heifer calves during the pre-weaning period.Frontiers in microbiology · 2024Article
- The microbiome of the pregnant uterus in Holstein dairy heifers and cows assessed by bacterial culture and 16S ribosomal RNA gene sequencing.Frontiers in microbiology · 2024Article
- Establishment of the uterine microbiome following artificial insemination in virgin heifers.Frontiers in microbiology · 2024Article
- Holistic View and Novel Perspective on Ruminal and Extra-Gastrointestinal Methanogens in Cattle.Microorganisms · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foetus sterility until parturition is under debate due to reports of microorganisms in the foetal environment and meconium. Sufficient controls to overcome sample contamination and provide direct evidence of microorganism viability in the pre-rectal gastrointestinal tract (GIT) have been lacking. We conducted molecular and culture-based analyses to investigate the presence of a microbiome in the foetal GIT of calves at 5, 6 and 7 months gestation, while controlling for contamination. The 5 components of the GIT (ruminal fluid, ruminal tissue, caecal fluid, caecal tissue and meconium) and amniotic fluid were found to contain a pioneer microbiome of distinct bacterial and archaeal communities. Bacterial and archaeal richness varied between GIT components. The dominant bacterial phyla in amniotic fluid differed to those in ruminal and caecal fluids and meconium. The lowest bacterial and archaeal abundances were associated with ruminal tissues. Viable bacteria unique to the ruminal fluids, which were not found in the controls from 5, 6 and 7 months gestation, were cultured, subcultured, sequenced and identified. We report that the foetal GIT is not sterile but is spatially colonised before birth by a pioneer microbiome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.