Evidence map›Paper›PMID 33077803›Full record

ArticleScientific reports2020

Geometric morphometric wing analysis represents a robust tool to identify female mosquitoes (Diptera: Culicidae) in Germany.

F G Sauer, L Jaworski, L Erdbeer, A Heitmann, J Schmidt-Chanasit, E Kiel, R Lühken

Abstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
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  3. Morphological and Genetic Heterogeneity inEcology and evolution · 2025
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  11. Microbiology spectrum · 2024
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  12. Article
  13. Article
  14. Article
  15. Species Discrimination ofAnimals : an open access journal from MDPI · 2023
    Article
  16. Article
  17. Article
  18. Intraspecific variation in wing geometry amongFrontiers in veterinary science · 2022
    Article
  19. Article
  20. Wing Morphometrics ofInsects · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

F G SauerAquatic Ecology and Nature Conservation, Carl von Ossietzky University Oldenburg, 26111, Oldenburg, Germany. felix.sauer@uol.de.
L JaworskiAquatic Ecology and Nature Conservation, Carl von Ossietzky University Oldenburg, 26111, Oldenburg, Germany.
L ErdbeerAquatic Ecology and Nature Conservation, Carl von Ossietzky University Oldenburg, 26111, Oldenburg, Germany.
A HeitmannBernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, 20359, Hamburg, Germany.
J Schmidt-ChanasitBernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, 20359, Hamburg, Germany.
E KielAquatic Ecology and Nature Conservation, Carl von Ossietzky University Oldenburg, 26111, Oldenburg, Germany.
R LühkenBernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, 20359, Hamburg, Germany. renkeluhken@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate species identification is the prerequisite to assess the relevance of mosquito specimens, but is often hindered by missing or damaged morphological features. The present study analyses the applicability of wing geometric morphometrics as a low-cost and practical alternative to identify native mosquitoes in Germany. Wing pictures were collected for 502 female mosquitoes of five genera and 19 species from 80 sampling sites. The reliable species identification based on interspecific wing geometry of 18 landmarks per specimen was tested. Leave-one-out cross validation revealed an overall accuracy of 99% for the genus and 90% for the species identification. Misidentifications were mainly due to three pairings of Aedes species: Aedes annulipes vs. Aedes cantans, Aedes cinereus vs. Aedes rossicus and Aedes communis vs. Aedes punctor. Cytochrome oxidase subunit I (COI) gene region was sequenced to validate the morphological and morphometric identification. Similar to the results of the morphometric analysis, the same problematic three Aedes-pairs clustered, but most other species could be well separated. Overall, our study underpins that morphometric wing analysis is a robust tool for reliable mosquito identification, which reach the accuracy of COI barcoding.

Indexed as

AedesAnimalsCulicidaeDNA Barcoding, TaxonomicElectron Transport Complex IVFemaleGermanyWings, AnimalElectron Transport Complex IV

Identifiers

PMID33077803
PMCPMC7573584

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.