ArticleProceedings of the National Academy of Sciences of the United States of America2020
Three-dimensional deconvolution processing for STEM cryotomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cancellation of Spectral and Spatial Crosstalk in Spectral Imaging for High-Dynamic-Range Electrophoretic Analysis of STR-PCR Products.Analytical chemistry · 2025Article
- Deconvolution to restore cryo-EM maps with anisotropic resolution.Research square · 2025Article
- Deconvolution to restore cryo-EM maps with anisotropic resolution.bioRxiv : the preprint server for biology · 2025Article
- Helical coiled nucleosome chromosome architectures during cell cycle progression.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Bringing Structure to Cell Biology with Cryo-Electron Tomography.Annual review of biophysics · 2023Review
- The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.Nature communications · 2023Article
- A proposed unified interphase nucleus chromosome structure: Preliminary preponderance of evidence.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A proposed unified mitotic chromosome architecture.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells.Journal of structural biology · 2022Article
- Entropy-regularized deconvolution of cellular cryotransmission electron tomograms.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A cryo-TSEM with temperature cycling capability allows deep sublimation of ice to uncover fine structures in thick cells.Scientific reports · 2021Article
- Single-particle cryo-EM: alternative schemes to improve dose efficiency.Journal of synchrotron radiation · 2021Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The complex environment of biological cells and tissues has motivated development of three-dimensional (3D) imaging in both light and electron microscopies. To this end, one of the primary tools in fluorescence microscopy is that of computational deconvolution. Wide-field fluorescence images are often corrupted by haze due to out-of-focus light, i.e., to cross-talk between different object planes as represented in the 3D image. Using prior understanding of the image formation mechanism, it is possible to suppress the cross-talk and reassign the unfocused light to its proper source post facto. Electron tomography based on tilted projections also exhibits a cross-talk between distant planes due to the discrete angular sampling and limited tilt range. By use of a suitably synthesized 3D point spread function, we show here that deconvolution leads to similar improvements in volume data reconstructed from cryoscanning transmission electron tomography (CSTET), namely a dramatic in-plane noise reduction and improved representation of features in the axial dimension. Contrast enhancement is demonstrated first with colloidal gold particles and then in representative cryotomograms of intact cells. Deconvolution of CSTET data collected from the periphery of an intact nucleus revealed partially condensed, extended structures in interphase chromatin.
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