ArticleBiomedical engineering online2020
Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters.
Article in Biomedical engineering online, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Review
- Microbial Transcription Factor-Based Biosensors: Innovations from Design to Applications in Synthetic Biology.Biosensors · 2025Review
- PlomBOX: a low cost bioassay for the sensitive detection of lead in drinking water.Communications engineering · 2025Article
- Novel yeast-based biosensor for environmental monitoring of tebuconazole.Applied microbiology and biotechnology · 2024Article
- At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring.ACS synthetic biology · 2023Article
- Review
- At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring.bioRxiv : the preprint server for biology · 2023Article
- Transcription factor-based biosensors for screening and dynamic regulation.Frontiers in bioengineering and biotechnology · 2023Review
- Synthetic biology techniques to tackle heavy metal pollution and poisoning.Synthetic and systems biotechnology · 2022Review
- Microbial Acoustical Analyzer for Antibiotic Indication.Sensors (Basel, Switzerland) · 2022Article
- Assessment of heavy metal contamination in herbal medicinal products consumed in the Iranian market.Environmental science and pollution research international · 2021Article
Corrections and comments
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Authors and funding
13 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.
methodsWe have designed and evaluated a bacterial biosensor expressing a luciferase reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing the CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr biosensor and pGL3-luc/cad biosensor, that were based on the expression of luc+ and under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.
resultsWe found that the pGL3-luc/pbr biosensor may be used to measure lead concentrations between 1-100 μM in the presence of other metals, including zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The pGL3-luc/cad biosensor could detect lead concentrations between 10 nM to 10 μM.
conclusionsThis biosensor was found to be specific for measuring lead ions in both environmental and biological samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.