ReviewGenes2020
Cytokinesis-Block Micronucleus Cytome Assay Evolution into a More Comprehensive Method to Measure Chromosomal Instability.
Review in Genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 1 synthesis or guideline pooled it, 137 citations in OpenAlex.
- Association Between Maternal Diet and Frequency of Micronuclei in Mothers and Newborns: A Systematic Review.Nutrients · 2025Pooled it
- Induction of aneuploidy by the metal-chelating agent neodecanoid acid in mammalian cells in vitro.Archives of toxicology · 2026Article
- Lymphocyte Micronucleus Formation Is Driven by Inflammation-Induced Oxidative DNA Damage in Oesophageal Cancer Development.International journal of cancer · 2026Article
- Antibiotic-Induced Genotoxicity: Molecular Mechanisms, Cytogenetic Damage, and Implications for Human Health.International journal of molecular sciences · 2026Review
- Synthesis, Characterization, and Genotoxic and Cytotoxic In Vitro Evaluation of Ceramic Nanoparticles of Sc Oxide Powders and Aerogels Doped with Europium Ions.Gels (Basel, Switzerland) · 2026Article
- Synthesis and characterization of chitosan-functionalized nanostructured lipid carriers with temozolomide: cytotoxic effects and chromosomal instability in human glioblastoma cells.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Article
- From In Vitro Genotoxicity Assays to Human Biomarkers: Interpreting DNA Damage Signals in Long-Term SSRI Therapy through a Translational Neurotoxicology Lens.Neurotoxicity research · 2026Review
- Casein-Lecithin Nanoemulsions Co-Encapsulating Vitamin E and Carvacrol as Multifunctional Edible Coatings for Meat Preservation.Gels (Basel, Switzerland) · 2026Article
- Micronuclei: origins, assays, mechanisms, diseases and treatments.Signal transduction and targeted therapy · 2026Review
- Boric Acid Prevents MMC- and H₂O₂-Related DNA Damage: Evidence from Cytogenetic and Comet Assays.Biological trace element research · 2026Article
- Micronucleus Assay in Human Lymphocytes and Exfoliated Buccal Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- In vitro metabolomics approaches to decipher the biochemical and cellular response to genotoxic compounds.EFSA journal. European Food Safety Authority · 2025Article
- Biopolymeric Films from the White Rind of Watermelon (ACS omega · 2025Article
- Toxicological Assessment ofInternational journal of molecular sciences · 2025Article
- The In Vitro Toxicity Profile of Nanoencapsulated Curcumin in a Chitosan-Alginate Gel Carrier Using Rabbit Lymphocytes: Preliminary Research.International journal of molecular sciences · 2025Article
- Unclassified Chromosomal Abnormalities as an Indicator of Genomic Damage in Survivors of Hodgkin's Lymphoma.Cancers · 2025Article
- Evaluation of the Cytotoxicity, Genotoxicity and Acute Oral Toxicity ofPharmaceuticals (Basel, Switzerland) · 2025Article
- Article
- Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This review describes the cytokinesis-block micronucleus (CBMN) cytome assay and its evolution into a molecular cytogenetic method of chromosomal instability (CIN). Micronuclei (MNi) originate from whole chromosomes or chromosome fragments that fail to segregate to the poles of the cell during mitosis. These lagging chromosomes are excluded from the daughter nuclei and are enveloped in their own membrane to form MNi. The CBMN assay was developed to allow MNi to be scored exclusively in once-divided binucleated cells, which enables accurate measurement of chromosome breakage or loss without confounding by non-dividing cells that cannot express MNi. The CBMN assay can be applied to cell lines in vitro and cells such as lymphocytes that can be stimulated to divide ex vivo. In the CBMN assay, other CIN biomarkers such as nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) are also measured. Use of centromere, telomere, and chromosome painting probes provides further insights into the mechanisms through which MNi, NPBs and NBUDs originate. Measurement of MNi is also important because entrapment within a micronucleus may cause chromosomes to shatter and, after nuclear reintegration, become rearranged. Additionally, leakage of DNA from MNi can stimulate inflammation via the cyclic GMP-AMP Synthase-Stimulator of Interferon Genes (cGAS-STING) DNA sensing mechanism of the innate immune system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.