Evidence map›Paper›PMID 33075709›Full record

ArticleVirology2021

Generation and preliminary characterization of vertebrate-specific replication-defective Zika virus.

Shengfeng Wan, Shengbo Cao, Xugang Wang, Yanfei Zhou, Weidong Yan, Xinbin Gu, Tzyy-Choou Wu, Xiaowu Pang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Shengfeng WanState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Department of Oral Pathology, College of Dentistry, Howard University, Washington, DC, 20059, USA; Department of Nephrology, Henan Provincial Key Laboratory of Kidney Disease and Immunology, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou, 450003, China.
Shengbo CaoState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Xugang WangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Yanfei ZhouTengen Biomedical Co., Rockville, MD, USA.
Weidong YanState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Department of Oral Pathology, College of Dentistry, Howard University, Washington, DC, 20059, USA.
Xinbin GuDepartment of Oral Pathology, College of Dentistry, Howard University, Washington, DC, 20059, USA.
Tzyy-Choou WuDepartment of Molecular Microbiology & Immunology, Johns Hopkins Medical Institutions, Baltimore, MD, 21287, USA.
Xiaowu PangDepartment of Oral Pathology, College of Dentistry, Howard University, Washington, DC, 20059, USA. Electronic address: xpang@howard.edu.
Howard University · USHuazhong Agricultural University · CNJohns Hopkins University · US

Funding

Train-Edu-Prog, Collaborative Cancer Research Training and Education Program Leading to Minority InitiativeP20CA192989 · NCI · HOWARD UNIVERSITY · PI MUSTAPHA, INDRA Z · 2014 to 2017
$801k
Development of Zika viral pseudoinfectious virus as Zika vaccine candidateR41AI129119 · NIAID · TENGEN BIOMEDICAL CO. · PI PANG, XIAOWU, ZHOU, YANFEI · 2017 to 2021
$652k
NCI NIH HHS P20 CA192989NIAID NIH HHS R41 AI129119
6 · The paper itself

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus that replicates in both vertebrate and insect cells, whereas insect-specific flaviviruses (ISF) replicate only in insect cells. We sought to convert ZIKV, from a dual-tropic flavivirus, into an insect-specific virus for the eventual development of a safe ZIKV vaccine. Reverse genetics was used to introduce specific mutations into the furin cleavage motif within the ZIKV pre-membrane protein (prM). Mutant clones were selected, which replicated well in C6/36 insect cells but exhibited reduced replication in non-human primate (Vero) cells. Further characterization of the furin cleavage site mutants indicated they replicated poorly in both human (HeLa, U251), and baby hamster kidney (BHK-21) cells. One clone with the induced mutation in the prM protein and at positions 291and 452 within the NS3 protein was totally and stably replication-defective in vertebrate cells (VSRD-ZIKV). Preliminary studies in ZIKV sensitive, immunodeficient mice demonstrated that VSRD-ZIKV-infected mice survived and were virus-negative. Our study indicates that a reverse genetic approach targeting the furin cleavage site in prM can be used to select an insect-specific ZIKV with the potential utility as a vaccine strain.

Indexed as

Virus ReplicationAnimalsCell LineChlorocebus aethiopsCricetinaeFurinHeLa CellsHost SpecificityHumansInsectaIsoquinolinesMembrane ProteinsMiceMutationReverse GeneticsVero CellsFurinfuro(2,3-c)isoquinolineIsoquinolinesMembrane ProteinsViral Nonstructural ProteinsViral ProteinsFurinprM cleavageVaccineVertebrate-specific replication-defectiveViral tropismZika virus

Identifiers

PMID33075709
PMCPMC7733535
OpenAlexW3092429904

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.