ArticlePloS one2020
Challenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 11 citations in OpenAlex.
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- Zoonotic bacteria in invasive California Kingsnake Lampropeltis californiae from Gran Canaria, Canary Islands, Spain.PloS one · 2025Article
- Staphylococci, Reptiles, Amphibians, and Humans: What Are Their Relations?Pathogens (Basel, Switzerland) · 2024Review
- Establishment of a Real-Time PCR Assay for the Detection ofAnimals : an open access journal from MDPI · 2023Article
- Trypanosoma cruzi DTU II coinfection with bacteria producing prolonged cutaneous lesion in a healthy young male.Revista do Instituto de Medicina Tropical de Sao Paulo · 2023Article
- Comparative Genomics of the Genus Pseudomonas Reveals Host- and Environment-Specific Evolution.Microbiology spectrum · 2022Article
- Activity of bacteria isolated from bats against Pseudogymnoascus destructans in China.Microbial biotechnology · 2022Article
- Comparative Genomics ofmSystems · 2021Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionBacterial pathogens are often involved in dermatitis in reptiles. Exact identification of reptile-specific but otherwise uncommon bacterial species may be challenging. However, identification is crucial to evaluate the importance of the detected bacterial species.
objectiveThe aim of this study was to assess the number of aerobic bacterial isolates cultured from skin-derived samples of reptiles which were not reliably identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), and to determine their identity. MATERIAL AND
methodsRoutine bacterial diagnostics were performed on 235 skin samples, and 417 bacterial isolates were analysed by MALDI-TOF MS. The isolates were grouped into categories based on their first score: category I (≥ 2.00), category II (≥ 1.70 and < 2.00), and category III (< 1.70). Isolates from category III were further investigated by 16S rRNA gene sequencing and the following criteria were applied: query cover 100%, e-value rounded to 0.0 and sequence identity (%) > 98.00% for genus identification, and > 99.00% for species identification.
resultsThe majority of bacterial isolates were in category I (85.1%) or category II (8.4%). In category III (6.5%) results achieved at first by MALDI-TOF MS corresponded to the results of the molecular analysis in 8.0% of isolates at the species level and in 24.0% at the genus level. Bacterial isolates classified as category III were heterogenic in genus (e.g. Chryseobacterium, Devriesea, Pseudomonas, Staphylococcus, Uruburuella), and some have only been described in reptiles so far.
conclusionsMost of the aerobic bacterial isolates cultured from reptile skin achieved high scores by MALDI-TOF MS. However, in the majority of category III isolates MALDI-TOF MS results were different from those of the molecular analysis. This strengthens the need to carefully examine low-scored results for plausibility and to be familiar with the occurrence and morphology of relevant reptile-specific bacterial species (e.g. Devriesea agamarum) as well as with the limits of the database used.
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