The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division.

Junko Otsuki, Toshiroh Iwasaki, Noritoshi Enatsu, Yuya Katada, Kohyu Furuhashi, Masahide Shiotani

Open access · goldAbstract readObservational Study

In one paragraph

Observational study in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 90% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Junko OtsukiAssisted Reproductive Technology Center, Okayama University, Okayama, Japan.ORCID 0000-0002-8183-8651

Toshiroh IwasakiHanabusa Women's Clinic, Kobe, Hyogo, Japan.

Noritoshi EnatsuHanabusa Women's Clinic, Kobe, Hyogo, Japan.

Yuya KatadaHanabusa Women's Clinic, Kobe, Hyogo, Japan.

Kohyu FuruhashiHanabusa Women's Clinic, Kobe, Hyogo, Japan.

Masahide ShiotaniHanabusa Women's Clinic, Kobe, Hyogo, Japan.

Kobe Women's University · JPOkayama University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The fate of the ICM in humans is still unknown, due to the ethical difficulties surrounding experimentation in this field. In this study we have explored the existing time-lapse recording data of embryos in the early stages of development, taking advantage of the large refractile bodies (RBs) within blastomeres as cellular markers. Our study found that the cellular composition of the ICM in humans is largely determined at the time of the fourth division and blastomeres which cleave first to fourth, during the fourth division from 8 cells to 16 cells, have the potential to be incorporated in the ICM.

Indexed as

Blastocyst Inner Cell MassBlastomeresCell DivisionEmbryonic DevelopmentFemaleHumansPregnancyReproductive Techniques, AssistedRetrospective StudiesTime-Lapse ImagingVideo Recording

Identifiers

PMID33075059

DOI10.1371/journal.pone.0240936

PMCPMC7571684

OpenAlexW3092955949

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.