Evidence map›Paper›PMID 33074545›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

RNA Interference on Regenerating Holothurian Gut Tissues.

Miosotis Alicea-Delgado, Samir A Bello-Melo, José E García-Arrarás

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Regeneration in Echinoderms: Molecular Advancements.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Miosotis Alicea-DelgadoUniversity of Puerto Rico, San Juan, Puerto Rico. aliceadelgadom@gmail.com.
Samir A Bello-MeloUniversity of Puerto Rico, San Juan, Puerto Rico.
José E García-ArrarásUniversity of Puerto Rico, San Juan, Puerto Rico.

Funding

Roadmap for Intestinal RegenerationR15GM124595 · NIGMS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI JOSE E GARCIA-ARRARAS · 2017 to 2026
$1.7M
Balancing degeneration and regeneration in the echinoderm nervous systemR21AG057974 · NIA · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI GARCIA-ARRARAS, JOSE E · 2017 to 2019
$646k
NIA NIH HHS R21 AG057974NIGMS NIH HHS R15 GM124595
6 · The paper itself

Abstract

Functional studies on echinoderms have been reduced to the use of pharmacological treatments. The ability to modulate the genetic expression of regenerating tissues can elucidate potential effectors during this process. Here we describe an effective transfection protocol that allows the introduction of Dicer-substrate interference RNAs (DsiRNAs) for the modulation of gene expression and its characterization during regeneration.

Indexed as

RNA InterferenceAnimalsElectroporationGene Expression RegulationRegenerationRNA, Small InterferingSea CucumbersTransfectionRNA, Small InterferingDsiRNAEchinodermElectroporationGut rudimentRegenerationRNAiSea Cucumber

Identifiers

PMID33074545
PMCPMC9503136

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.