ArticleMethods in molecular biology (Clifton, N.J.)2021
Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Molecular evidence for early deuterostome origins of ovarian cell types and neuroendocrine control of reproduction.Nature communications · 2026Article
- Deep conservation of cis-regulatory elements and chromatin organization in echinoderms uncover ancestral regulatory features of animal genomes.Nature ecology & evolution · 2026Article
- A collagenous extracellular matrix regulates germline gene expression in the sea star embryo.Scientific reports · 2025Article
- Article
- A molecular basis for spine color morphs in the sea urchin Lytechinus variegatus.Scientific reports · 2024Article
- Characterization of thyrotropin-releasing hormone producing neurons in sea urchin, from larva to juvenile.Frontiers in neuroscience · 2024Article
- microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development.Developmental biology · 2023Article
- Article
- Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ.Nature communications · 2023Article
- Gene regulatory divergence amongst echinoderms underlies appearance of pigment cells in sea urchin development.Developmental biology · 2023Article
- Article
- Article
- Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis.Experimental & applied acarology · 2022Article
- A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.Developmental biology · 2022Article
- Ovothiol ensures the correct developmental programme of the sea urchinOpen biology · 2022Article
- Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins.Frontiers in cell and developmental biology · 2022Article
- FISH for All: A Fast and Efficient FluorescentFrontiers in physiology · 2022Article
- Polarized Dishevelled dissolution and reassembly drives embryonic axis specification in sea star oocytes.Current biology : CB · 2021Article
- Single-cell RNA sequencing of theeLife · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Identifying the location of a specific RNA in a cell, tissue, or embryo is essential to understand its function. Here we use echinoderm embryos to demonstrate the power of fluorescence in situ RNA hybridizations to localize sites of specific RNA accumulation in whole mount embryo applications. We add to this technology the use of various probe-labeling technologies to colabel multiple RNAs in one application and we describe protocols for incorporating immunofluorescence approaches to maximize the information obtained in situ. We offer alternatives for these protocols and troubleshooting advice to identify steps in which the procedure may have failed. Overall, echinoderms are wonderfully suited for these technologies, and these protocols are applicable to a wide range of cells, tissues, and embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.