Evidence map›Paper›PMID 33073910›Full record

ArticleCell proliferation2020

Detection of SARS-CoV-2 in saliva and characterization of oral symptoms in COVID-19 patients.

Lili Chen, Jiajia Zhao, Jinfeng Peng, Xiaoshuang Li, Xuliang Deng, Zhi Geng, Zhenyu Shen, Fengyuan Guo, Qianwen Zhang, Yang Jin and 2 more

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06272019 (Alternative Application Methods of Pre-procedural Povidone Iodine Mouthrinse), which is not on this map. Cited by 161 papers, 10 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
161citing papers in PubMed, 10 pooled it
37.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06272019 nacompletednot on this mapstarted 2023, after this paper: background citation

Alternative Application Methods of Pre-procedural Povidone Iodine Mouthrinse

TypeinterventionalSponsorChulalongkorn UniversityRan2023 to 2024Enrolled25ConditionsThe Retention of Iodine in Saliva Over the Time, The Comparison of Iodine in Saliva Between Gargle and SwabArmsIodine gargle
3 · Its place in the literature

Who cites it

161 citing papers in PubMed, 10 syntheses or guidelines pooled it, 277 citations in OpenAlex.

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  4. Oral Manifestations of COVID-19 in Hospitalized Patients: A Systematic Review.International journal of environmental research and public health · 2021
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101 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Lili ChenDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.ORCID https://orcid.org/0000-0002-9965-8990
Jiajia ZhaoDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Jinfeng PengDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.ORCID https://orcid.org/0000-0002-7438-3337
Xiaoshuang LiDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xuliang DengDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Zhi GengDepartment of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Zhenyu ShenDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Fengyuan GuoDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Qianwen ZhangDepartment of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Yang JinDepartment of Respiratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Lin WangDepartment of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Songlin WangSalivary Gland Disease Center and Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.
Union Hospital · CNCapital Medical University · CNPeking University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesIn order to provide a more comprehensive understanding of the effects of SARS-CoV-2 on oral health and possible saliva transmission, we performed RNA-seq profiles analysis from public databases and also a questionnaire survey on oral-related symptoms of COVID-19 patients. MATERIALS AND

methodsTo analyse ACE2 expression in salivary glands, bulk RNA-seq profiles from four public datasets including 31 COVID-19 patients were recruited. Saliva and oropharyngeal swabs were collected. SARS-CoV-2 nucleic acids in saliva were detected by real-time polymerase chain reaction (RT-PCR). Additionally, a questionnaire survey on various oral symptoms such as dry mouth and amblygeustia was also carried out on COVID-19 patients.

resultsACE2 expression was present at detectable levels in the salivary glands. In addition, of four cases with positive detection of salivary SARS-CoV-2 nucleic acids, three (75%) were critically ill on ventilator support. Furthermore, we observed the two major oral-related symptoms, dry mouth (46.3%) and amblygeustia (47.2%), were manifested by a relatively high proportion of 108 COVID-19 patients who accepted the questionnaire survey.

conclusionsThis study confirms the expression of ACE2 in the salivary glands and demonstrates the possibility of SARS-CoV-2 infection of salivary glands. Saliva may be a new source of diagnostic specimens for critically ill patients, since it can be easily collected without any invasive procedures. In addition, dry mouth and amblygeustia can be considered as initial symptoms of COVID-19 infection.

Indexed as

Angiotensin-Converting Enzyme 2COVID-19FemaleHumansMaleReal-Time Polymerase Chain ReactionSalivaSARS-CoV-2ACE2 protein, humanAngiotensin-Converting Enzyme 2amblygeustiaCOVID-19 patientsdry mouthsalivaSARS-CoV-2

Identifiers

PMID33073910
PMCPMC7645955
OpenAlexW3014006087

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.