Evidence map›Paper›PMID 33072921›Full record

ArticleHeliyon2020

Experimental design of a culture approach for corneal endothelial cells of New Zealand white rabbit.

María Dolores Montalvo-Parra, Isaac Alejandro Vidal-Paredes, Cesar E Calzada-Rodríguez, Italia Tatnaí Cárdenas-Rodríguez, Guiomar Farid Torres-Guerrero, Daniela Gómez-Elizondo, Mariana López-Martínez, Judith Zavala, Jorge E Valdez-García

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

María Dolores Montalvo-ParraTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Isaac Alejandro Vidal-ParedesTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Cesar E Calzada-RodríguezTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Italia Tatnaí Cárdenas-RodríguezTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Guiomar Farid Torres-GuerreroTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Daniela Gómez-ElizondoTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Mariana López-MartínezTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Judith ZavalaTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.
Jorge E Valdez-GarcíaTecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, 3000 Morones Prieto Ave., Col. Los Doctores, Monterrey, N.L., Mexico.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The harvesting of corneal endothelial cells (CEC) has received special attention due to its potential as a therapy for corneal blindness. The main challenges are related to the culture media formulation, cellular density at the primary isolation, and the number of passages in which CEC can retain their functional characteristics. To alternate different media formulations to harvest CEC has an impact on the cellular yield and morphology. Therefore, we analyzed four different sequences of growth factor-supplemented Stimulatory (S) and non-supplemented Quiescent (Q) media, upon passages to find the optimal S-Q culture sequence. We assessed cell yield, morphology, procollagen I production, Na

Indexed as

ATPaseCell biologyCell cultureCell differentiationCircularityCollagenCorneal endotheliumExperimental designExtracellular matrixOphthalmologyRegenerative medicineTissue cultureTissue engineering

Identifiers

PMID33072921
PMCPMC7548448

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.