Evidence map›Paper›PMID 33072854›Full record

ArticleAdvanced materials technologies2020

Flow Homogenization Enables a Massively Parallel Fluidic Design for High-throughput and Multiplexed Cell Isolation.

Chinchun Ooi, Christopher M Earhart, Casey E Hughes, Jung-Rok Lee, Dawson J Wong, Robert J Wilson, Rajat Rohatgi, Shan X Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials technologies, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Chinchun OoiDepartment of Chemical Engineering, Stanford University, Stanford, California, USA; Department of Fluid Dynamics, Institute of High Performance Computing, Singapore.
Christopher M EarhartDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.
Casey E HughesDivision of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.
Jung-Rok LeeDivision of Mechanical and Biomechanical Engineering, Ewha Womans University, Seoul, South Korea.
Dawson J WongDepartment of Electrical Engineering, Stanford University, Stanford, California, USA.
Robert J WilsonDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.
Rajat RohatgiDepartment of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.
Shan X WangDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA; Department of Electrical Engineering, Stanford University, Stanford, California, USA.
Stanford University · USEwha Womans University · KRInstitute of High Performance Computing · SGUniversity of Utah · US

Funding

Pilot ProjectsU54CA151459 · NCI · STANFORD UNIVERSITY · PI GAMBHIR, SANJIV S · 2010 to 2014
$12.6M
Nano Characterization and Nano Fabrication CoreU54CA199075 · NCI · STANFORD UNIVERSITY · PI SINCLAIR, ROBERT · 2015 to 2019
$9.6M
Cancer Sample Preparation with Micromachined Magnetic Sifter and NanoparticlesR33CA138330 · NCI · STANFORD UNIVERSITY · PI WAKELEE, HEATHER ANN, WANG, SHAN X. · 2010 to 2012
$738k
NCI NIH HHS R33 CA138330NCI NIH HHS U54 CA151459NCI NIH HHS U54 CA199075
6 · The paper itself

Abstract

Microfluidic devices are widely used for applications such as cell isolation. Currently, the most common method to improve throughput for microfluidic devices involves fabrication of multiple, identical channels in parallel. However, this 'numbering up' only occurs in one dimension, thereby limiting gains in volumetric throughput. In contrast, macro-fluidic devices permit high volumetric flow-rates but lack the finer control of microfluidics. Here, we demonstrate how a micro-pore array design enables flow homogenization across a magnetic cell capture device, thus creating a massively parallel series of micro-scale flow channels with consistent fluidic and magnetic properties, regardless of spatial location. This design enables scaling in 2-dimensions, allowing flow-rates exceeding 100 mL/hr while maintaining >90% capture efficiencies of spiked lung cancer cells from blood in a simulated circulating tumor cell system. Additionally, this design facilitates modularity in operation, which we demonstrate by combining two different devices in tandem for multiplexed cell separation in a single pass with no additional cell losses from processing.

Indexed as

flow homogenizationmagnetic separationmicrofluidicsmultiplexed cell separationrare cell isolation

Identifiers

PMID33072854
PMCPMC7567302
OpenAlexW3011678418

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.