ArticlePlant methods2020
Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Validated reference genes for normalization of RT-qPCR in developing organs of wheat to study developmentally/spatio-temporally expressed family genes.Scientific reports · 2025Article
- Lipid remodeling and response mechanisms during the germination of aged oat seeds.BMC plant biology · 2025Article
- Identification of reference genes for gene expression assessment in Avena sativa under biotic stress triggered by Blumeria graminis.Scientific reports · 2024Article
- Exploring Evolutionary Pathways and Abiotic Stress Responses through Genome-Wide Identification and Analysis of the Alternative Oxidase (AOX) Gene Family in Common Oat (International journal of molecular sciences · 2024Article
- The Contribution ofFoods (Basel, Switzerland) · 2024Article
- Differential expression and global analysis of miR156/SQUAMOSA promoter binding-like proteins (SPL) module in oat.Scientific reports · 2024Article
- Genome-Wide Analysis of the Oat (International journal of molecular sciences · 2024Article
- Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets.Plants (Basel, Switzerland) · 2023Article
- Characterization of the Metallothionein Gene Family inAntioxidants (Basel, Switzerland) · 2023Article
- Avenanthramides, Distinctive Hydroxycinnamoyl Conjugates of Oat,Plants (Basel, Switzerland) · 2023Review
- Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought.Scientific reports · 2023Article
- Genome-wide analysis and expression profiling of glyoxalase gene families in oat (Frontiers in plant science · 2023Article
- LncRNAs elevate plant adaptation under low temperature by maintaining local chromatin landscape.Plant signaling & behavior · 2022Review
- Reference genes expression stability in Avena sativa L. during compatible and incompatible interactions with Puccinia graminis.Scientific reports · 2022Article
- Validation of Reference Genes in a Population of BlueberryPlants (Basel, Switzerland) · 2022Article
- Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (International journal of molecular sciences · 2022Article
- Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.).Scientific reports · 2022Article
- Dynamic Responses of Antioxidant and Glyoxalase Systems to Seed Aging Based on Full-Length Transcriptome in Oat (Antioxidants (Basel, Switzerland) · 2022Article
- Identification of Viruses Infecting Oats in Korea by Metatranscriptomics.Plants (Basel, Switzerland) · 2022Article
- Validation of Reference Genes for Studying Different Abiotic Stresses in Oat (Plants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOat (
resultsIn this study, eleven candidate RGs, including four duplicated genes, were selected from oat transcriptome. The stability and the optimal combination of these candidate RGs were assessed in 18 oat samples by using four statistical algorithms including the ΔCt method, geNorm, NormFinder and BestKeeper. The most stable RGs for "all samples", "shoots and roots of seedlings", "developing seeds" and "developing endosperms" were
conclusionsResults provide a proof of concept that the duplicated RG is feasible for qPCR in polyploids. To our knowledge, this study is the first systematic research on the optimal RGs for accurate qPCR normalization of gene expression in different organs and tissues of oat.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.