ArticleReproductive medicine and biology2020
Abundance of cell-free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts.
Article in Reproductive medicine and biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- "Old and new" frontiers in embryo viability assessment: current practices and introduction of emerging approaches.Biology of reproduction · 2026Review
- Non-invasive embryo assessment: Cell-free DNA-based genetic testing and amino acid metabolomics in relation to morphology: A case-control study.International journal of reproductive biomedicine · 2026Article
- Mitochondrial DNA to genomic DNA ratio in embryo spent culture medium as a predictive marker for in vitro fertilization outcomes: a pilot study.Journal of assisted reproduction and genetics · 2026Article
- Review
- Ploidy and Implantation Potential: Non-Invasive Small Non-Coding RNA-Based Health Assessment of Day 5 and 6 Blastocysts.International journal of molecular sciences · 2025Article
- The role of mitochondrial DNA copy number in spent culture medium in predicting outcomes of single blastocyst transfer.Journal of assisted reproduction and genetics · 2025Article
- Relative mtDNA copy number in embryo spent culture medium is not a reliable biomarker of human embryo aneuploidy.Reproduction & fertility · 2025Article
- Machine learning in time-lapse imaging to differentiate embryos from young vs old mice†.Biology of reproduction · 2024Article
- Elevated cell-free mitochondria DNA level of patients with premature ovarian insufficiency.BMC pregnancy and childbirth · 2023Article
- An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo.Current genomics · 2022Review
- Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos-Impact on Infertility Outcome.Current issues in molecular biology · 2022Article
- Resveratrol enhanced mitochondrial recovery from cryopreservation-induced damages in oocytes and embryos.Reproductive medicine and biology · 2021Review
- Embryo and Its Mitochondria.Antioxidants (Basel, Switzerland) · 2021Review
- Abundance of cell-free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts.Reproductive medicine and biology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture medium (SCM) of human-expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf-mtDNA in SCM could predict the quality of blastocysts.
methodsEmbryos (n = 53) were individually cultured in a time-lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf-mtDNA in SCM (20 μL) of expanded blastocysts were determined using real-time PCR.
resultsThe duration between start of blastulation to expanded blastocyst (tEB-tSB) and between that of the blastocyst stage to expanded blastocyst (tEB-tB) significantly and positively correlated with the abundance of cf-mtDNA in the SCM (tEB-tSB: r = .46;
conclusionsThe abundance of cf-mtDNA in the SCM was associated with expansion duration and BC. Thus, cf-mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.