ArticleReproductive medicine and biology2020
Improvement of early developmental competence of postovulatory-aged oocytes using metaphase II spindle injection in mice.
Article in Reproductive medicine and biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- Reprogramming the aging ovarian microenvironment via mitochondrial sharing and structural remodeling.Theranostics · 2025Article
- Nuclear transfer leads to aberrant cell cycle regulation and translation, as determined by gene expression, and selection of mitochondrial DNA in porcine blastocysts.NAR molecular medicine · 2024Article
- In vivo and in vitro postovulatory aging: when time works against oocyte quality?Journal of assisted reproduction and genetics · 2022Review
- Patient-tailored reproductive health care.Frontiers in reproductive health · 2022Review
- Improvement of early developmental competence of postovulatory-aged oocytes using metaphase II spindle injection in mice.Reproductive medicine and biology · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
purposeAssisted reproductive technology (ART) is a widely applied fertility treatment. However, the developmental competence of aged oocytes from women of a late reproductive age is seriously reduced and the aged oocytes often fail in fertilization even when ART is used. To resolve this problem, we examined usefulness of a new method "the metaphase II spindle transfer (MESI)" as ART using mouse oocytes.
methodsThis work was composed of two experiments. First, 24 hours after collection, embryos from oocytes (1-day-old oocytes, called postovulatory-aged oocytes), were observed, after intracytoplasmic sperm injection (ICSI), and it was found that they were not able to reach the blastocyst stage. Next, the metaphase II chromosome-spindle complexes from 1-day-old oocytes were injected into cytoplasts from oocytes just collected, using piezo pulses to generate reconstructed oocytes. This procedure was named metaphase II spindle injection (MESI).
resultsAfter ICSI, embryos from the reconstructed oocytes (32/105), which contained the genes of 1-day-old oocytes, were able to develop into the blastocyst stage. The fragmentation rate after ICSI was 28.6%. Thus, the developmental competence of 1-day-old oocytes was improved by MESI.
conclusionsThe MESI method has the potential to improve the success rate of infertility treatments for women of a late reproductive age.
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