Evidence map›Paper›PMID 33070223›Full record

ArticleJournal of assisted reproduction and genetics2021

Treatment with Laevo (L)-carnitine reverses the mitochondrial function of human embryos.

Naoharu Morimoto, Shu Hashimoto, Masaya Yamanaka, Manabu Satoh, Yoshiharu Nakaoka, Atsushi Fukui, Yoshiharu Morimoto, Hiroaki Shibahara

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Naoharu MorimotoIVF Namba Clinic, Osaka, 550-0015, Japan.
Shu HashimotoGraduate School of Medicine, Osaka City University, Osaka, 545-8585, Japan. hashimoto@ivfnamba.com.ORCID http://orcid.org/0000-0001-9083-1403
Masaya YamanakaIVF Namba Clinic, Osaka, 550-0015, Japan.
Manabu SatohIVF Namba Clinic, Osaka, 550-0015, Japan.
Yoshiharu NakaokaIVF Namba Clinic, Osaka, 550-0015, Japan.
Atsushi FukuiDepartment of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
Yoshiharu MorimotoHORAC Grand Front Osaka Clinic, Osaka, 530-0011, Japan.
Hiroaki ShibaharaDepartment of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
Hyogo Medical University · JPOsaka City University · JPOsaka Nishi Clinic · JP

Funding

Japan Agency for Medical Research and Development 17gk0110014h0002Japan Agency for Medical Research and Development 18gk0110014h0003Japan Society for the Promotion of Science 17K08144Japan Society for the Promotion of Science 20K09674
6 · The paper itself

Abstract

purposeLaevo (l)-carnitine plays important roles in reducing the cytotoxic effects of free fatty acids by forming acyl-carnitine and promoting beta-oxidation, leading to alleviation of cell damage. Recently, the mitochondrial functions in morula has been shown to decrease with the maternal age. Here, we assessed the effect of l-carnitine on mitochondrial function in human embryos and embryo development.

methodsTo examine the effect of L-carnitine on mitochondrial function in morulae, 38 vitrified-thawed embryos at the 6-11-cell stage on day 3 after ICSI were donated from 19 couples. Each couple donated two embryos. Two siblings from each couple were divided randomly into two groups and were cultured in medium with or without 1 mM L-carnitine. The oxygen consumption rates (OCRs) were measured at morula stage. The development of 1029 zygotes cultured in medium with or without L-carnitine was prospectively analyzed.

resultsAddition of L-carnitine to the culture medium significantly increased the OCRs of morulae and improved the morphologically-good blastocyst formation rate per zygote compared with sibling embryos. Twenty healthy babies were born from embryos cultured in L-carnitine-supplemented medium after single embryo transfers. CONCLUSION(S): L-carnitine is a promising culture medium supplement that might be able to counteract the decreased mitochondrial function in human morula stage embryos.

Indexed as

BlastocystCarnitineCulture MediaEmbryo Culture TechniquesEmbryo, MammalianEmbryonic DevelopmentFemaleHumansMitochondriaOxidation-ReductionOxygen ConsumptionSingle Embryo TransferZygoteCarnitineCulture MediaLaevo (L)-carnitineMitochondrial functionOxygen consumption rate

Identifiers

PMID33070223
PMCPMC7822972
OpenAlexW3092896272

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.