ArticleEuropean journal of cell biology2020
Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes.
Article in European journal of cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Live-Cell SOFI Correlation with SMLM and AFM Imaging.ACS bio & med chem Au · 2023Article
- Extracellular matrix assembly stress initiates Drosophila central nervous system morphogenesis.Developmental cell · 2023Article
- Measuring the Co-Localization and Dynamics of Mobile Proteins in Live Cells Undergoing Signaling Responses.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages.iScience · 2022Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Podosomes are mechanosensitive attachment/invasion structures that form on the matrix-adhesion interface of cells and protrude into the extracellular matrix to probe and remodel. Despite their central role in many cellular processes, their exact molecular structure and function remain only partially understood. We review recent progress in molecular scale imaging of podosome architecture, including our newly developed localisation microscopy technique termed HAWK which enables artefact-free live-cell super-resolution microscopy of podosome ring proteins, and report new results on combining fluorescence localisation microscopy (STORM/PALM) and atomic force microscopy (AFM) on one setup, where localisation microscopy provides the location and dynamics of fluorescently labelled podosome components, while the spatial variation of stiffness is mapped with AFM. For two-colour localisation microscopy we combine iFluor-647, which has previously been shown to eliminate the need to change buffer between imaging modes, with the photoswitchable protein mEOS3.2, which also enables live cell imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.