ArticleBioorganic & medicinal chemistry2020
Genetic code expansion in mammalian cells: A plasmid system comparison.
Article in Bioorganic & medicinal chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Efficient and Site-Specific Protein Bioconjugation Quantification in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations.Nucleic acids research · 2025Article
- Strategies to Expand the Genetic Code of Mammalian Cells.Chemical reviews · 2025Review
- Aligning fermentation conditions with non-canonical amino acid addition strategy is essential for Nε-((2-azidoethoxy)carbonyl)-L-lysine uptake and incorporation into the target protein.Scientific reports · 2024Article
- Genetically Encoded Lysine Analogues with Differential Light Sensitivity for Activation of Protein Function.ChemPhotoChem · 2024Article
- Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery.Cell reports methods · 2023Article
- Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion.Journal of the American Chemical Society · 2023Article
- Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation.ACS omega · 2023Article
- Visible light-induced specific protein reaction delineates early stages of cell adhesion.bioRxiv : the preprint server for biology · 2023Article
- Chemical Biology Approaches to Identify and Profile Interactors of Chromatin Modifications.ACS chemical biology · 2023Review
- Genetic Encoding of a Photocaged Histidine for Light-Control of Protein Activity.Chembiochem : a European journal of chemical biology · 2023Article
- Genetic code expansion to enable site-specific bioorthogonal labeling of functional G protein-coupled receptors in live cells.Protein science : a publication of the Protein Society · 2023Article
- Quantitative Analysis and Optimization of Site-Specific Protein Bioconjugation in Mammalian Cells.Bioconjugate chemistry · 2022Article
- Enhanced incorporation of subnanometer tags into cellular proteins for fluorescence nanoscopy via optimized genetic code expansion.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Efficient Amber SuppressionACS synthetic biology · 2022Article
- Optical Control of Phosphoinositide Binding: Rapid Activation of Subcellular Protein Translocation and Cell Signaling.ACS synthetic biology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Genetic code expansion with unnatural amino acids (UAAs) has significantly broadened the chemical repertoire of proteins. Applications of this method in mammalian cells include probing of molecular interactions, conditional control of biological processes, and new strategies for therapeutics and vaccines. A number of methods have been developed for transient UAA mutagenesis in mammalian cells, each with unique features and advantages. All have in common a need to deliver genes encoding additional protein biosynthetic machinery (an orthogonal tRNA/tRNA synthetase pair) and a gene for the protein of interest. In this study, we present a comparative evaluation of select plasmid-based genetic code expansion systems and a detailed analysis of suppression efficiency with different UAAs and in different cell lines.
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