Evidence map›Paper›PMID 33069136›Full record

ArticleBiomaterials2021

Trb3 controls mesenchymal stem cell lineage fate and enhances bone regeneration by scaffold-mediated local gene delivery.

Jiabing Fan, Chung-Sung Lee, Soyon Kim, Xiao Zhang, Joan Pi-Anfruns, Mian Guo, Chen Chen, Matthew Rahnama, Jiong Li, Benjamin M Wu and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes.Redox report : communications in free radical research · 2024
    Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Jiabing FanDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA; Weintraub Center for Reconstructive Biotechnology, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Chung-Sung LeeDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Soyon KimDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Xiao ZhangDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Joan Pi-AnfrunsDivision of Diagnostic and Surgical Sciences, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Mian GuoDepartment of Neurosurgery, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China.
Chen ChenDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Matthew RahnamaDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Jiong LiDepartment of Medicinal Chemistry, Institute for Structural Biology, Drug Discovery and Development, Philips Institute for Oral Health Research, Virginia Commonwealth University, Richmond, VA, 23298, USA.
Benjamin M WuDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA; Weintraub Center for Reconstructive Biotechnology, School of Dentistry, University of California, Los Angeles, CA, 90095, USA; Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Tara L AghalooDivision of Diagnostic and Surgical Sciences, School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
Min LeeDivision of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA, 90095, USA; Weintraub Center for Reconstructive Biotechnology, School of Dentistry, University of California, Los Angeles, CA, 90095, USA; Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA. Electronic address: leemin@ucla.edu.
University of California, Los Angeles · USHarbin Medical University · CNVirginia Commonwealth University · US

Funding

Tribbles homolog 3 and BMP-2 induced bone formationR01DE027332 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Tara L Aghaloo, Min Lee · 2018 to 2026
$2.7M
NIDCR NIH HHS R01 DE027332
6 · The paper itself

Abstract

Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.

Indexed as

Mesenchymal Stem CellsAdipogenesisBone RegenerationCell DifferentiationCell LineageOsteogenesisAdipogenesisGelCA-PLGA ScaffoldMSCOsteogenesisTrb3

Identifiers

PMID33069136
PMCPMC7655726
OpenAlexW3093022078

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.