ArticleAnimal reproduction science2020
RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa.
Article in Animal reproduction science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Heat Stress and the Bull Sperm Transcriptome: Molecular Mechanisms, Fertility, and Epigenetic Inheritance.Animals : an open access journal from MDPI · 2026Review
- Review
- Comparative transcriptome analysis of bull X- and Y-spermatozoa.Scientific reports · 2025Article
- Comparative gene expression profiling of fertility related transcripts in bull sperm of different genetic backgrounds.Tropical animal health and production · 2025Article
- Relation between proteome characterization and semen quality in Italian chicken breeds.PloS one · 2025Article
- Silent cells? Potential for context-dependent gene expression in mature sperm.Proceedings. Biological sciences · 2025Review
- RNA Sequencing of Sperm from Healthy Cattle and Horses Reveals the Presence of a Large Bacterial Population.Current issues in molecular biology · 2024Article
- Article
- Integrative Bioinformatics Analysis of The Cell Division Cycle and Ribosomal Pathways in The Rat Varicocele: Implications for Drug Discovery.Cell journal · 2023Article
- Integrating sperm cell transcriptome and seminal plasma metabolome to analyze the molecular regulatory mechanism of sperm motility in Holstein stud bulls.Journal of animal science · 2023Article
- Integrated multi-omics analyses reveals molecules governing sperm metabolism potentially influence bull fertility.Scientific reports · 2022Article
- Sperm Transcripts Associated With Odorant Binding and Olfactory Transduction Pathways Are Altered in Breeding Bulls Producing Poor-Quality Semen.Frontiers in veterinary science · 2022Article
- Identification of differentially expressed mRNAs and miRNAs in spermatozoa of bulls of varying fertility.Frontiers in veterinary science · 2022Article
- The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point.Frontiers in endocrinology · 2022Article
- Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common CarpInternational journal of molecular sciences · 2021Article
- Comparative Transcriptomic Analysis of Spermatozoa From High- and Low-Fertile Crossbred Bulls: Implications for Fertility Prediction.Frontiers in cell and developmental biology · 2021Article
- Cellular and Molecular Insights Into the Etiology of Subfertility/Infertility in Crossbred Bulls (Frontiers in cell and developmental biology · 2021Review
- Significance and Relevance of Spermatozoal RNAs to Male Fertility in Livestock.Frontiers in genetics · 2021Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm, which are believed to be transcriptionally and translationally inactive, synthesize RNA and proteins before there is gradual disappearance of the ribosome during chromatin compaction. Sperm transfer several functionally relevant transcripts to the oocyte, controlling maternal-zygotic transition and embryonic development. The present study was undertaken to profile and analyze sperm transcripts comprehensively using Next Generation Ribonucleic acid sequencing technology in Holstein Friesian x Tharparkar crossbred bulls. The results from global transcriptomic profiling revealed transcripts for 13,814 genes; of which 431 transcripts were expressed with >1 FPKM and 13,383 transcripts were expressed with >0 or <1 FPKM. The abundant mRNA transcripts of crossbred bull sperm were PRM1 and HMGB4. Gene ontology of transcripts with>1 FPKM revealed there was a major involvement in the structural constituent of ribosomes and translation. Results from pathway enrichment indicated the connection between ribosome, oxidative phosphorylation and spliceosome pathways and the transcripts of crossbred bull spermatozoa. The transcriptional abundance of selected genes, validated using RT-qPCR, indicated significant variations between bulls. Collectively, it may be inferred that the transcripts in crossbred bull sperm were heavily implicated in functions such as the structural constituent of ribosomes and translation, and pathways such as ribosome, oxidative phosphorylation and spliceosome. Further studies using larger sample sizes are required to understand the possible implications of transcriptomic variations on semen quality and fertility.
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