Evidence map›Paper›PMID 33068532›Full record

ArticleNeuron2020

Emergence of Neuronal Diversity during Vertebrate Brain Development.

Bushra Raj, Jeffrey A Farrell, Jialin Liu, Jakob El Kholtei, Adam N Carte, Joaquin Navajas Acedo, Lucia Y Du, Aaron McKenna, Đorđe Relić, Jessica M Leslie and 1 more

Open access · bronzeAbstract read
In one paragraph

Article in Neuron, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 126 citations in OpenAlex.

  1. Article
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  8. PALINCODE: Recording cell lineage with ternary palindromic CRISPR bits.bioRxiv : the preprint server for biology · 2026
    Article
  9. Article
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  11. GlialeLife · 2026
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  16. The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Article
  17. A conserved logic for the development of cortical layering in tetrapods.bioRxiv : the preprint server for biology · 2025
    Article
  18. Article
  19. Article
  20. Article

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 3 countries.

Bushra RajDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA. Electronic address: bushranraj@gmail.com.
Jeffrey A FarrellDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Unit on Cell Specification and Differentiation, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20814, USA.
Jialin LiuAllen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA; Biozentrum, University of Basel, 4056 Basel, Switzerland.
Jakob El KholteiAllen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA; Biozentrum, University of Basel, 4056 Basel, Switzerland.
Adam N CarteDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Biozentrum, University of Basel, 4056 Basel, Switzerland; Systems, Synthetic, and Quantitative Biology Program, Harvard University, Cambridge, MA 02138, USA.
Joaquin Navajas AcedoAllen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA; Biozentrum, University of Basel, 4056 Basel, Switzerland.
Lucia Y DuAllen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA; Biozentrum, University of Basel, 4056 Basel, Switzerland.
Aaron McKennaDepartment of Molecular and Systems Biology, Dartmouth Geisel School of Medicine, Lebanon, NH 03756, USA.
Đorđe RelićBiozentrum, University of Basel, 4056 Basel, Switzerland; Swiss Institute of Bioinformatics (SIB), 4056 Basel, Switzerland.
Jessica M LeslieDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Alexander F SchierDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA; Biozentrum, University of Basel, 4056 Basel, Switzerland; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA; Center for Brain Science, Harvard University, Cambridge, MA 02138, USA. Electronic address: alex.schier@unibas.ch.
Harvard University · USUniversity of Basel · CHBroad Institute · USDartmouth College · USSIB Swiss Institute of Bioinformatics · CH

Funding

DNA-mediated recording of cellular historyDP1HD094764 · NICHD · HARVARD UNIVERSITY · PI SCHIER, ALEXANDER F · 2017 to 2019
$3.5M
Embryonic gene regulatory networks from spatially resolved transcriptomesR01HD085905 · NICHD · HARVARD UNIVERSITY · PI SCHIER, ALEXANDER F · 2016 to 2019
$2.6M
Multidimensional cell recording with single-cell genomicsR00HG010152 · NHGRI · DARTMOUTH COLLEGE · PI MCKENNA, AARON H · 2019 to 2021
$729k
Global investigation of cell trajectory and lineage relationships in the vertebrate brain with single-cell transcriptomicsK99HD098298 · NICHD · HARVARD UNIVERSITY · PI RAJ, BUSHRA · 2019 to 2020
$257k
Error Correction in Early EmbryosK99HD091291 · NICHD · HARVARD UNIVERSITY · PI FARRELL, JEFFREY ALLEN · 2017 to 2018
$255k
Multidimensional cell recording with single-cell genomicsK99HG010152 · NHGRI · UNIVERSITY OF WASHINGTON · PI MCKENNA, AARON H · 2018 to 2018
$97k
CIHRNHGRI NIH HHS K99 HG010152NHGRI NIH HHS R00 HG010152NICHD NIH HHS DP1 HD094764NICHD NIH HHS K99 HD091291NICHD NIH HHS K99 HD098298NICHD NIH HHS R01 HD085905
6 · The paper itself

Abstract

Neurogenesis comprises many highly regulated processes including proliferation, differentiation, and maturation. However, the transcriptional landscapes underlying brain development are poorly characterized. We describe a developmental single-cell catalog of ∼220,000 zebrafish brain cells encompassing 12 stages from embryo to larva. We characterize known and novel gene markers for ∼800 clusters and provide an overview of the diversification of neurons and progenitors across these time points. We also introduce an optimized GESTALT lineage recorder that enables higher expression and recovery of Cas9-edited barcodes to query lineage segregation. Cell type characterization indicates that most embryonic neural progenitor states are transitory and transcriptionally distinct from neural progenitors of post-embryonic stages. Reconstruction of cell specification trajectories reveals that late-stage retinal neural progenitors transcriptionally overlap cell states observed in the embryo. The zebrafish brain development atlas provides a resource to define and manipulate specific subsets of neurons and to uncover the molecular mechanisms underlying vertebrate neurogenesis.

Indexed as

AnimalsBrainCell DifferentiationCell LineageGene Expression Regulation, DevelopmentalNeurogenesisNeuronsZebrafishbraincell specificationCRISPR-Cas9 barcodinglineageneurogenesisprogenitorretinascRNA-Seqtranscriptional cascadezebrafish

Identifiers

PMID33068532
PMCPMC8286448
OpenAlexW3093029727

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.