ArticleJournal of industrial microbiology & biotechnology2020
Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw material.
Article in Journal of industrial microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- CRISPR-Mediated Metabolic Engineering ofMicroorganisms · 2026Article
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- Engineering an AlkS-PalkB Transcription Factor-Based Biosensor With Improved Sensitivity to Isobutanol and Other Short-Chain Alcohols.Microbial biotechnology · 2025Article
- Improving sustainable isopropanol production in engineered Escherichia coli W via oxygen limitation.Microbial cell factories · 2025Article
- Microbial host engineering for sustainable isobutanol production from renewable resources.Applied microbiology and biotechnology · 2024Review
- Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca.Microbial cell factories · 2024Article
- An efficient approach for overproduction of DNA polymerase from Pyrococcus furiosus using an optimized autoinduction system in Escherichia coli.World journal of microbiology & biotechnology · 2024Article
- Engineering new-to-nature biochemical conversions by combining fermentative metabolism with respiratory modules.Nature communications · 2024Article
- EngineeringMicroorganisms · 2023Article
- Growth-coupled anaerobic production of isobutanol from glucose in minimal medium with Escherichia coli.Biotechnology for biofuels and bioproducts · 2023Article
- A fluorescent reporter system for anaerobic thermophiles.Frontiers in bioengineering and biotechnology · 2023Article
- System metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The aim of this study was to establish isobutanol production on chemically defined medium in Escherichia coli. By individually expressing each gene of the pathway, we constructed a plasmid library for isobutanol production. Strain screening on chemically defined medium showed successful production in the robust E. coli W strain, and expression vector IB 4 was selected as the most promising construct due to its high isobutanol yields and efficient substrate uptake. The investigation of different aeration strategies in combination with strain improvement and the implementation of a pulsed fed-batch were key for the development of an efficient production process. E. coli W ΔldhA ΔadhE Δpta ΔfrdA enabled aerobic isobutanol production at 38% of the theoretical maximum. Use of cheese whey as raw material resulted in longer process stability, which allowed production of 20 g l
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.