Evidence map›Paper›PMID 33067741›Full record

ArticleJournal of assisted reproduction and genetics2021

Ageing and ovarian stimulation modulate the relative levels of transcript abundance of oocyte DNA repair genes during the germinal vesicle-metaphase II transition in mice.

Fabrizzio Horta, Aravind Ravichandran, Sally Catt, Beverley Vollenhoven, Peter Temple-Smith

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PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
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  8. BRCA Mutations and Fertility Preservation.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Fabrizzio HortaEducation Program in Reproduction and Development, EPRD, Department of Obstetrics and Gynecology, School of Clinical Science, Monash University, Melbourne, VIC, 3168, Australia. Fabrizzio.Horta@monash.edu.ORCID http://orcid.org/0000-0003-3212-4924
Aravind RavichandranEducation Program in Reproduction and Development, EPRD, Department of Obstetrics and Gynecology, School of Clinical Science, Monash University, Melbourne, VIC, 3168, Australia.
Sally CattEducation Program in Reproduction and Development, EPRD, Department of Obstetrics and Gynecology, School of Clinical Science, Monash University, Melbourne, VIC, 3168, Australia.
Beverley VollenhovenEducation Program in Reproduction and Development, EPRD, Department of Obstetrics and Gynecology, School of Clinical Science, Monash University, Melbourne, VIC, 3168, Australia.
Peter Temple-SmithEducation Program in Reproduction and Development, EPRD, Department of Obstetrics and Gynecology, School of Clinical Science, Monash University, Melbourne, VIC, 3168, Australia.
Monash University · AUMonash Health · AUMonash IVF · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeOocyte quality and reproductive outcome are negatively affected by advanced maternal age, ovarian stimulation and method of oocyte maturation during assisted reproduction; however, the mechanisms responsible for these associations are not fully understood. The aim of this study was to compare the effects of ageing, ovarian stimulation and in-vitro maturation on the relative levels of transcript abundance of genes associated with DNA repair during the transition of germinal vesicle (GV) to metaphase II (MII) stages of oocyte development.

methodsThe relative levels of transcript abundance of 90 DNA repair-associated genes was compared in GV-stage and MII-stage oocytes from unstimulated and hormone-stimulated ovaries from young (5-8-week-old) and old (42-45-week-old) C57BL6 mice. Ovarian stimulation was conducted using pregnant mare serum gonadotropin (PMSG) or anti-inhibin serum (AIS). DNA damage response was quantified by immunolabeling of the phosphorylated histone variant H2AX (γH2AX).

resultsThe relative transcript abundance in DNA repair genes was significantly lower in MII oocytes compared to GV oocytes in young unstimulated and PMSG stimulated but was higher in AIS-stimulated mice. Interestingly, an increase in the relative level of transcript abundance of DNA repair genes was observed in MII oocytes from older mice in unstimulated, PMSG-stimulated and AIS-stimulated mice. Decreased γH2AX levels were found in both GV oocytes (82.9%) and MII oocytes (37.5%) during ageing in both ovarian stimulation types used (PMSG/AIS; p < 0.05).

conclusionsIn conclusion, DNA repair relative levels of transcript abundance are altered by maternal age and the method of ovarian stimulation during the GV-MII transition in oocytes.

Indexed as

AgingAnimalsDNA DamageDNA RepairFemaleGene Expression Regulation, DevelopmentalGonadotropins, EquineHistonesHumansInhibinsMetaphaseMiceOocytesOogenesisOvulation InductionPregnancygamma-H2AX protein, mouseGonadotropins, EquineHistonesInhibinsFemale ageingIn vitro maturationmRNAOocyte DNA repairOvarian stimulation

Identifiers

PMID33067741
PMCPMC7822980
OpenAlexW3093147926

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.