Evidence map›Paper›PMID 33067686›Full record

ReviewEuropean biophysics journal : EBJ2020

Analytical ultracentrifuge: an ideal tool for characterization of non-coding RNAs.

Maulik D Badmalia, M Quadir Siddiqui, Tyler Mrozowich, Darren L Gemmill, Trushar R Patel

Abstract readReview
PubMed Publisher
In one paragraph

Review in European biophysics journal : EBJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maulik D BadmaliaDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Dr. West, Lethbridge, AB, T1K 3M4, Canada.ORCID http://orcid.org/0000-0002-3384-6550
M Quadir SiddiquiDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Dr. West, Lethbridge, AB, T1K 3M4, Canada.ORCID http://orcid.org/0000-0003-0891-3122
Tyler MrozowichDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Dr. West, Lethbridge, AB, T1K 3M4, Canada.ORCID http://orcid.org/0000-0002-1077-9682
Darren L GemmillDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Dr. West, Lethbridge, AB, T1K 3M4, Canada.ORCID http://orcid.org/0000-0003-2834-9063
Trushar R PatelDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Dr. West, Lethbridge, AB, T1K 3M4, Canada. trushar.patel@uleth.ca.ORCID http://orcid.org/0000-0003-0627-2923

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Analytical ultracentrifugation (AUC) has emerged as a robust and reliable technique for biomolecular characterization with extraordinary sensitivity. AUC is widely used to study purity, conformational changes, biomolecular interactions, and stoichiometry. Furthermore, AUC is used to determine the molecular weight of biomolecules such as proteins, carbohydrates, and DNA and RNA. Due to the multifaceted role(s) of non-coding RNAs from viruses, prokaryotes, and eukaryotes, research aimed at understanding the structure-function relationships of non-coding RNAs is rapidly increasing. However, due to their large size, flexibility, complicated secondary structures, and conformations, structural studies of non-coding RNAs are challenging. In this review, we are summarizing the application of AUC to evaluate the homogeneity, interactions, and conformational changes of non-coding RNAs from adenovirus as well as from Murray Valley, Powassan, and West Nile viruses. We also discuss the application of AUC to characterize eukaryotic long non-coding RNAs, Xist, and HOTAIR. These examples highlight the significant role AUC can play in facilitating the structural determination of non-coding RNAs and their complexes.

Indexed as

RNA, UntranslatedRNA, ViralUltracentrifugationRNA, UntranslatedRNA, ViralAggregationAnalytical ultracentrifugeFlaviviral RNAsHomogeneityLong non-coding RNAsSedimentation coefficient

Identifiers

PMID33067686

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.