Evidence map›Paper›PMID 33067534›Full record

ArticleScientific reports2020

SPARC promotes insulin secretion through down-regulation of RGS4 protein in pancreatic β cells.

Li Hu, Fengli He, Meifeng Huang, Qian Zhao, Lamei Cheng, Neveen Said, Zhiguang Zhou, Feng Liu, Yan-Shan Dai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Lack of CFTR alters the ferret pancreatic ductal epithelial secretome and cellular proteome: Implications for exocrine/endocrine signaling.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2022
    Article
  12. Article
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Li HuDepartment of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Fengli HeDepartment of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Meifeng HuangDepartment of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Qian ZhaoInstitute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan, China.
Lamei ChengInstitute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan, China.
Neveen SaidDepartment of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Zhiguang ZhouDepartment of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Feng LiuDepartment of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Yan-Shan DaiDepartment of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China. Ydai88@csu.edu.cn.
Central South University · CNSecond Xiangya Hospital of Central South University · CNWake Forest University · US

Funding

Regulation of Urinary Bladder Carcinogenesis and Progression by SPARCR01CA193437 · NCI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI SAID, NEVEEN · 2016 to 2020
$1.8M
NCI NIH HHS R01 CA193437
6 · The paper itself

Abstract

SPARC-deficient mice have been shown to exhibit impaired glucose tolerance and insulin secretion, but the underlying mechanism remains unknown. Here, we showed that SPARC enhanced the promoting effect of Muscarinic receptor agonist oxotremorine-M on insulin secretion in cultured mouse islets. Overexpression of SPARC down-regulated RGS4, a negative regulator of β-cell M3 muscarinic receptors. Conversely, knockdown of SPARC up-regulated RGS4 in Min6 cells. RGS4 was up-regulated in islets from sparc -/- mice, which correlated with decreased glucose-stimulated insulin secretion (GSIS). Furthermore, inhibition of RGS4 restored GSIS in the islets from sparc -/- mice, and knockdown of RGS4 partially decreased the promoting effect of SPARC on oxotremorine-M-stimulated insulin secretion. Phosphoinositide 3-kinase (PI3K) inhibitor LY-294002 abolished SPARC-induced down-regulation of RGS4. Taken together, our data revealed that SPARC promoted GSIS by inhibiting RGS4 in pancreatic β cells.

Indexed as

AnimalsDiabetes Mellitus, ExperimentalGlucoseGlucose IntoleranceHumansInsulinInsulin-Secreting CellsInsulin SecretionIslets of LangerhansMiceMice, Inbred C57BLMice, KnockoutOsteonectinOxotremorinePhosphatidylinositol 3-KinasesReceptor, Muscarinic M3GlucoseInsulinOsteonectinOxotremorineoxotremorine MPhosphatidylinositol 3-KinasesReceptor, Muscarinic M3RGS4 proteinRGS ProteinsSPARC protein, humanSPARC protein, mouse

Identifiers

PMID33067534
PMCPMC7567887
OpenAlexW3093002105

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.