ArticleScientific reports2020
Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Plastics as vectors for pathogens and antibiotic resistance genes in aquatic systems.Water science and technology : a journal of the International Association on Water Pollution Research · 2026Review
- Fungal DNA profiles across maternal and neonatal biological sites: findings from an exploratory pilot study.Frontiers in microbiology · 2026Article
- Disseminated ascariasis infection with encephalopathy: Molecular confirmation and phylogenetic insights.IDCases · 2026Article
- A review on microbial-biofilm mediated mechanisms in marine microplastics degradation.Antonie van Leeuwenhoek · 2025Review
- Rapid Colonisation of Plastic Surfaces by Marine Alcanivorax Bacteria Is Flagellum-Dependent and Influenced by Polymer Type and Photo-Weathering State.Environmental microbiology · 2025Article
- Zooming in the plastisphere: the ecological interface for phytoplankton-plastic interactions in aquatic ecosystems.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Article
- Oceanic regions shape the composition of the Antarctic plastisphere.Communications earth & environment · 2025Article
- Community composition and seasonal dynamics of microplastic biota in the Eastern Mediterranean Sea.Scientific reports · 2024Article
- What Quality Suffices for Nanopore Metabarcoding? Reconsidering Methodology and Ectomycorrhizae in DecayingJournal of fungi (Basel, Switzerland) · 2024Article
- Primed and ready: nanopore metabarcoding can now recover highly accurate consensus barcodes that are generally indel-free.BMC genomics · 2024Article
- Wuchereria bancrofti Lymphatic Filariasis, Barrancabermeja, Colombia, 2023.Emerging infectious diseases · 2024Article
- A full-length 18S ribosomal DNA metabarcoding approach for determining protist community diversity using Nanopore sequencing.Ecology and evolution · 2024Article
- Assessing the Plastisphere from Floating Plastics in the Northwestern Mediterranean Sea, with Emphasis on Viruses.Microorganisms · 2024Article
- The first report of Dirofilaria repens infection in dogs from Colombia.Parasitology research · 2023Article
- Inter-comparison of marine microbiome sampling protocols.ISME communications · 2023Article
- Article
- Isolation and characterization of crude-oil-dependent bacteria from the coast of Ghana using oxford nanopore sequencing.Heliyon · 2023Article
- DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus.PloS one · 2023Article
- The geographical and seasonal effects on the composition of marine microplastic and its microbial communities: The case study of Israel and Portugal.Frontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastic debris in the ocean form a new ecosystem, termed 'plastisphere', which hosts a variety of marine organisms. Recent studies implemented DNA metabarcoding to characterize the taxonomic composition of the plastisphere in different areas of the world. In this study, we used a modified metabarcoding approach which was based on longer barcode sequences for the characterization of the plastisphere biota. We compared the microbiome of polyethylene food bags after 1 month at sea to the free-living biome in two proximal but environmentally different locations on the Mediterranean coast of Israel. We targeted the full 1.5 kb-long 16S rRNA gene for bacteria and 0.4-0.8 kb-long regions within the 18S rRNA, ITS, tufA and COI loci for eukaryotes. The taxonomic barcodes were sequenced using Oxford Nanopore Technology with multiplexing on a single MinION flow cell. We identified between 1249 and 2141 species in each of the plastic samples, of which 61 species (34 bacteria and 27 eukaryotes) were categorized as plastic-specific, including species that belong to known hydrocarbon-degrading genera. In addition to a large prokaryotes repertoire, our results, supported by scanning electron microscopy, depict a surprisingly high biodiversity of eukaryotes within the plastisphere with a dominant presence of diatoms as well as other protists, algae and fungi.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.