ArticleScientific reports2020
Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Immunotherapy for rapid bone marrow conditioning and leukemia depletion that allows efficient hematopoietic stem cell transplantation.Journal for immunotherapy of cancer · 2025Article
- Proteomic Insights into Human Limbal Epithelial Progenitor-Derived Small Extracellular Vesicles.Stem cell reviews and reports · 2025Article
- Multiple Stem/Progenitor Cells Isolated from the Limbus.Current stem cell research & therapy · 2025Review
- The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells.Frontiers in cell and developmental biology · 2025Article
- Article
- Beyond the Epidermal-Melanin-Unit: The Human Scalp Anagen Hair Bulb Is Home to Multiple Melanocyte Subpopulations of Variable Melanogenic Capacity.International journal of molecular sciences · 2023Article
- Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering.International journal of molecular sciences · 2023Article
- Enrichment of Human Dermal Stem Cells from Primary Cell Cultures through the Elimination of Fibroblasts.Cells · 2023Article
- Article
- Article
- Melanocyte stem cells in skin diseases and their potential in cell-based therapy.Histology and histopathology · 2022Review
- P-Cadherin Is Expressed by Epithelial Progenitor Cells and Melanocytes in the Human Corneal Limbus.Cells · 2022Article
- Identification, Isolation, and Characterization of Melanocyte Precursor Cells in the Human Limbal Stroma.International journal of molecular sciences · 2022Article
- Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus.International journal of molecular sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Limbal melanocytes (LM) are located in the basal epithelial layer of the corneoscleral limbus and interact with adjacent limbal epithelial progenitor cells. The exploration of their biological role in the maintenance of the limbal stem cell niche has been limited by the difficulty of LM isolation and cultivation. Here, we report on a facile protocol for the efficient isolation and enrichment of pure populations of human LMs by fluorescence-activated cell sorting (FACS) using antibodies raised against the cell surface marker CD117 (c-Kit). The enriched LMs retain self-renewal capacity and sustained melanin production, and are suitable to study the potential of LMs in stem cell-based corneal tissue engineering.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.