Evidence map›Paper›PMID 33067471›Full record

ArticleNature communications2020

DIALib-QC an assessment tool for spectral libraries in data-independent acquisition proteomics.

Mukul K Midha, David S Campbell, Charu Kapil, Ulrike Kusebauch, Michael R Hoopmann, Samuel L Bader, Robert L Moritz

Abstract readEvaluation Study
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mukul K MidhaInstitute for Systems Biology, Seattle, WA, 98109, USA.ORCID http://orcid.org/0000-0003-4053-0682
David S CampbellInstitute for Systems Biology, Seattle, WA, 98109, USA.
Charu KapilInstitute for Systems Biology, Seattle, WA, 98109, USA.ORCID http://orcid.org/0000-0002-8750-5006
Ulrike KusebauchInstitute for Systems Biology, Seattle, WA, 98109, USA.ORCID http://orcid.org/0000-0001-6162-7577
Michael R HoopmannInstitute for Systems Biology, Seattle, WA, 98109, USA.ORCID http://orcid.org/0000-0001-7029-7792
Samuel L BaderInstitute for Systems Biology, Seattle, WA, 98109, USA.
Robert L MoritzInstitute for Systems Biology, Seattle, WA, 98109, USA. rmoritz@systemsbiology.org.ORCID http://orcid.org/0000-0002-3216-9447

Funding

Systems BiologyU19AG023122 · NIA · TRANSLATIONAL GENOMICS RESEARCH INST · PI RICHARD A MILLER · 2004 to 2026
$102.6M
Shortening the development cycle time of Trans Proteomic Pipeline tools with high performance computingR01GM087221 · NIGMS · INSTITUTE FOR SYSTEMS BIOLOGY · PI DEUTSCH, ERIC, MORITZ, ROBERT L · 2010 to 2021
$6.0M
Novel peptide based biomarkers for Lyme disease diagnosticsR21AI133335 · NIAID · INSTITUTE FOR SYSTEMS BIOLOGY · PI MORITZ, ROBERT L · 2018 to 2019
$522k
NIAID NIH HHS R21 AI133335NIA NIH HHS U19 AG023122NIGMS NIH HHS R01 GM087221
6 · The paper itself

Abstract

Data-independent acquisition (DIA) mass spectrometry, also known as Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH), is a popular label-free proteomics strategy to comprehensively quantify peptides/proteins utilizing mass spectral libraries to decipher inherently multiplexed spectra collected linearly across a mass range. Although there are many spectral libraries produced worldwide, the quality control of these libraries is lacking. We present the DIALib-QC (DIA library quality control) software tool for the systematic evaluation of a library's characteristics, completeness and correctness across 62 parameters of compliance, and further provide the option to improve its quality. We demonstrate its utility in assessing and repairing spectral libraries for correctness, accuracy and sensitivity.

Indexed as

SoftwareHumansMass SpectrometryPeptide LibraryPeptidesProteinsProteomicsPeptide LibraryPeptidesProteins

Identifiers

PMID33067471
PMCPMC7567827

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.