ArticleScience advances2020
Imaging and quantifying homeostatic levels of intracellular silicon in diatoms.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- The morphogenetic activity of dAnk genes in the diatom Thalassiosira pseudonana is sensitive to Si availability.Communications biology · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Structural organization of p62 filaments and the cellular ultrastructure of calcium-rich p62-enwrapped lipid droplet cargo.Nature communications · 2025Article
- Increased aluminum exposure induces widespread changes in silicon, carbon, and nitrogen metabolism in Entomoneis vertebralis.BMC genomics · 2025Article
- Harnessing Microalgae as Sustainable Cell Factories for Polyamine-Based Nanosilica for Biomedical Applications.Molecules (Basel, Switzerland) · 2025Review
- Hexagonal Patterns in Diatom Silica Form via a Directional Two-Step Process.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Mechanism of branching morphogenesis inspired by diatom silica formation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Generating open-source 3D phytoplankton models by integrating photogrammetry with scanning electron microscopy.Frontiers in microbiology · 2024Article
- Rethinking Antigen Source: Cancer Vaccines Based on Whole Tumor Cell/tissue Lysate or Whole Tumor Cell.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Characterization of the molecular mechanisms of silicon uptake in coccolithophores.Environmental microbiology · 2023Article
- Phase Separation of Oppositely Charged Polymers Regulates Bioinspired Silicification.Angewandte Chemie (International ed. in English) · 2022Article
- Biomineralization: Integrating mechanism and evolutionary history.Science advances · 2022Review
- Diatoms in Volcanic Soils of Mutnovsky and Gorely Volcanoes (Kamchatka Peninsula, Russia).Microorganisms · 2021Article
- Structural evidence for extracellular silica formation by diatoms.Nature communications · 2021Article
- Article
- A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diatoms are an abundant group of microalgae, known for their ability to form an intricate cell wall made of silica. Silicon levels in seawater are in the micromolar range, making it a challenge for diatoms to supply the rapid intracellular silicification process with the needed flux of soluble silicon. Here, we use three-dimensional cryo-electron microscopy and spectroscopy to quantitatively analyze, at submicrometer spatial resolution and sensitivity in the millimolar range, intracellular silicon in diatom cells. Our results show that the internal silicon concentration inside the cell is ~150 mM in average, three orders of magnitude higher than the external environment. The cellular silicon content is not compartmentalized, but rather unevenly distributed throughout the cell. Unexpectedly, under silicon starvation, the internal silicon pool is not depleted, reminiscent of a constitutive metabolite. Our spatially resolved approach to analyze intracellular silicon opens avenues to investigate this homeostatic trait of diatoms.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.