ArticleInternational journal of molecular sciences2020
Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Extracellular Vesicles From Human Fallopian Tubes Enhance IVF Embryo Development and Contain Functional Proteins Including YWHAZ.Journal of extracellular vesicles · 2026Article
- Extracellular vesicles in bovine reproduction: their journey from gametogenesis to pregnancy.Frontiers in cell and developmental biology · 2026Review
- [The role of extracellular vesicles in early embryo development and their application in assisted reproductive technologies].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025Review
- Moxibustion Alleviates Inflammation via SIRT5-mediated Post-translational Modification and Macrophage Polarization.Inflammation · 2025Article
- Effects of hormone-primed oviduct epithelial cell co-culture system on swine SCNT embryo development.Frontiers in cell and developmental biology · 2025Article
- Deciphering embryo-maternal cross-talk: advanced in vitro approaches.Animal reproduction · 2025Article
- Oviduct epithelium interactions: roles in sperm selection and embryo quality.Animal reproduction · 2025Article
- Mir-218-5p from Extracellular Vesicles of Endometrium in Patients with Recurrent Implantation Failure Impairs Pre-Implantation Embryo Development.International journal of nanomedicine · 2025Article
- Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro.Bulletin of experimental biology and medicine · 2024Article
- Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early development of bovine in vitro fertilized embryos.The Journal of reproduction and development · 2024Article
- Extracellular vesicles affecting embryo developmentFrontiers in pharmacology · 2024Review
- Exosomes: New regulators of reproductive development.Materials today. Bio · 2023Review
- Extracellular vesicles from human Fallopian tubal fluid benefit embryo developmentHuman reproduction open · 2023Article
- Effect of estrous cycle phases on gene expression in bovine oviduct epithelial cells.Veterinary world · 2022Article
- Extracellular vesicles improve embryo cryotolerance by maintaining the tight junction integrity during blastocoel re-expansion.Reproduction (Cambridge, England) · 2022Article
- BOEC-Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos.Animals : an open access journal from MDPI · 2022Article
- Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances.Frontiers in cell and developmental biology · 2022Review
- Article
- The Complicated Effects of Extracellular Vesicles and Their Cargos on Embryo Implantation.Frontiers in endocrinology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Oviduct flushing is enriched by a wide variety of nutrients that guide the 3-4 days journey of pre-implantation embryo through the oviduct as it develops into a competent blastocyst (BL). However, little is known about the specific requirement and role of these nutrients that orchestrate the early stages of embryonic development. In this study, we aimed to characterize the effect of in vitro-derived bovine oviduct epithelial cell (BOECs) secretion that mimics the in vivo oviduct micro-fluid like environment, which allows successful embryonic development. In this study, the addition of an in vitro derived BOECs-condition media (CM) and its isolated exosomes (Exo) significantly enhances the quality and development of BL, while the hatching ability of BLs was found to be high (48.8%) in the BOECs-Exo supplemented group. Surprisingly, BOECs-Exo have a dynamic effect on modulating the embryonic metabolism by restoring the pyruvate flux into TCA-cycle. Our analysis reveals that Exo treatment significantly upregulates the pyruvate dehydrogenase (PDH) and glutamate dehydrogenase (GLUD1) expression, required for metabolic fine-tuning of the TCA-cycle in the developing embryos. Exo treatment increases the influx into TCA-cycle by strongly suppressing the PDH and GLUD1 upstream inhibitors, i.e., PDK4 and SIRT4. Improvement of TCA-cycle function was further accompanied by higher metabolic activity of mitochondria in BOECs-CM and Exo in vitro embryos. Our study uncovered, for the first time, the possible mechanism of BOECs-derived secretion in re-establishing the TCA-cycle flux by the utilization of available nutrients and highlighted the importance of pyruvate in supporting bovine in vitro embryonic development.
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Registered trials
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