ArticleMolecules (Basel, Switzerland)2020
Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection.
Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025Review
- Critical evaluation of the potential of ICP-MS-based systems in toxicological studies of metallic nanoparticles.Analytical and bioanalytical chemistry · 2024Review
- Physicochemical characterization and quantification of nanoplastics: applicability, limitations and complementarity of batch and fractionation methods.Analytical and bioanalytical chemistry · 2023Article
- The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery.Molecules (Basel, Switzerland) · 2023Review
- Electrical asymmetric-flow field-flow fractionation with a multi-detector array platform for the characterization of metallic nanoparticles with different coatings.Analytical and bioanalytical chemistry · 2023Article
- A comparative analysis of extracellular vesicles (EVs) from human and feline plasma.Scientific reports · 2022Article
- Characterization of Gold Nanorods Conjugated with Synthetic Glycopolymers Using an Analytical Approach Based on spICP-SFMS and EAF4-MALS.Nanomaterials (Basel, Switzerland) · 2021Article
- Influence of Physicochemical Characteristics and Stability of Gold and Silver Nanoparticles on Biological Effects and Translocation across an Intestinal Barrier-A Case Study from In Vitro to In Silico.Nanomaterials (Basel, Switzerland) · 2021Article
- Dealing with the complexity of conjugated and self-assembled polymer-nanostructures using field-flow fractionation.Analytical science advances · 2021Review
- Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers.Drug delivery and translational research · 2021Review
- Measuring particle concentration of multimodal synthetic reference materials and extracellular vesicles with orthogonal techniques: Who is up to the challenge?Journal of extracellular vesicles · 2021Article
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Authors and funding
6 authors.
Funding
Abstract
Accurate physico-chemical characterization of exosomes and liposomes in biological media is challenging due to the inherent complexity of the sample matrix. An appropriate purification step can significantly reduce matrix interferences, and thus facilitate analysis of such demanding samples. Electrical Asymmetrical Flow Field-Flow Fractionation (EAF4) provides online sample purification while simultaneously enabling access to size and Zeta potential of sample constituents in the size range of approx. 1-1000 nm. Hyphenation of EAF4 with Multi-Angle Light Scattering (MALS) and Nanoparticle Tracking Analysis (NTA) detection adds high resolution size and number concentration information turning this setup into a powerful analytical platform for the comprehensive physico-chemical characterization of such challenging samples. We here present EAF4-MALS hyphenated with NTA for the analysis of liposomes and exosomes in complex, biological media. Coupling of the two systems was realized using a flow splitter to deliver the sample at an appropriate flow speed for the NTA measurement. After a proof-of-concept study using polystyrene nanoparticles, the combined setup was successfully applied to analyze liposomes and exosomes spiked into cell culture medium and rabbit serum, respectively. Obtained results highlight the benefits of the EAF4-MALS-NTA platform to study the behavior of these promising drug delivery vesicles under in vivo like conditions.
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