Evidence map›Paper›PMID 33065285›Full record

ArticleActa biomaterialia2020

Scaffolds with controlled release of pro-mineralization exosomes to promote craniofacial bone healing without cell transplantation.

W Benton Swanson, Zhen Zhang, Kemao Xiu, Ting Gong, Miranda Eberle, Ziqi Wang, Peter X Ma

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
100citing papers in PubMed, 2 pooled it
7.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

100 citing papers in PubMed, 2 syntheses or guidelines pooled it, 167 citations in OpenAlex.

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40 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

W Benton SwansonDepartment of Biologic and Materials Science, School of Dentistry, University of Michigan, Ann Arbor, USA.
Zhen ZhangDepartment of Biologic and Materials Science, School of Dentistry, University of Michigan, Ann Arbor, USA.
Kemao XiuDepartment of Biologic and Materials Science, School of Dentistry, University of Michigan, Ann Arbor, USA.
Ting GongDepartment of Biologic and Materials Science, School of Dentistry, University of Michigan, Ann Arbor, USA.
Miranda EberleDepartment of Chemistry, University of Michigan, Ann Arbor, USA.
Ziqi WangDepartment of Mechanical Engineering, College of Engineering, University of Michigan, Ann Arbor, USA.
Peter X MaDepartment of Biologic and Materials Science, School of Dentistry, University of Michigan, Ann Arbor, USA; Macromolecular Science and Engineering Center, College of Engineering, University of Michigan, Ann Arbor, USA; Department of Biomedical Engineering, College of Engineering and Medical School, University of Michigan, Ann Arbor, USA; Department of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, USA. Electronic address: mapx@umich.edu.
University of Michigan · US

Funding

Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
PTH and Calcium Synergy for Craniofacial RegenerationR01DE022327 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MCCAULEY, LAURIE K. · 2012 to 2022
$3.8M
Regenerating cranial suture (Jaylynn Jones)R01DE027662 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HATCH, NAN E, MA, PETER X · 2018 to 2022
$2.6M
Exosome-mediated Craniofacial Bone Tissue Engineering by Controlled ReleaseF30DE029359 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SWANSON, WILLIAM BENTON · 2020 to 2024
$231k
NIDCR NIH HHS F30 DE029359NIDCR NIH HHS R01 DE022327NIDCR NIH HHS R01 DE027662NIDCR NIH HHS T32 DE007057
6 · The paper itself

Abstract

Biomimetic bone regeneration methods which demonstrate both clinical and manufacturing feasibility, as alternatives to autogenic or allogenic bone grafting, remain a challenge to the field of tissue engineering. Here, we report the pro-osteogenic capacity of exosomes derived from human dental pulp stem cells (hDPSCs) to facilitate bone marrow stromal cell (BMSC) differentiation and mineralization. To support their delivery, we engineered a biodegradable polymer delivery platform to improve the encapsulation and the controlled release of exosomes on a tunable time scale from poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) triblock copolymer microspheres. Our delivery platform integrates within three-dimensional tissue engineering scaffolds to enable a straightforward surgical insertion into a mouse calvarial defect. We demonstrate the osteogenic potential of these functional constructs in vitro and in vivo. Controlled release of osteogenic hDPSC-derived exosomes facilitates osteogenic differentiation of BMSCs, leading to mineralization to a degree which is comparable to exogenous administration of the same exosomes in human and mouse BMSCs. By recruiting endogenous cells to the defects and facilitating their differentiation, the controlled release of osteogenic exosomes from a tissue engineering scaffold demonstrates accelerated bone healing in vivo at 8 weeks. Exosomes recapitulate the advantageous properties of mesenchymal stem/progenitor cells, without manufacturing or immunogenic concerns associated with transplantation of exogenous cells. This biomaterial platform enables exosome-mediated bone regeneration in an efficacious and clinically relevant way.

Indexed as

ExosomesOsteogenesisAnimalsBone RegenerationCell DifferentiationCell TransplantationDelayed-Action PreparationsMiceTissue ScaffoldsDelayed-Action PreparationsControlled releaseDrug deliveryExosomeExtracellular vesicleIssue engineering

Identifiers

PMID33065285
PMCPMC7796555
OpenAlexW3093111519

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.